Pig soluble P-selectin,sP-Selectin ELISA Kit

Pig soluble P-selectin,sP-Selectin ELISA Kit

Product Details

Citations

Customer Reviews and Q&A

Product Details

Citations

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Customer Reviews and Q&A

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Pig soluble P-selectin,sP-Selectin ELISA Kit

Citations (1) Protocol MSDS

Code	CSB-E08106p
Size	96T,5×96T,10×96T
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Product Details
Citations (1)
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Target Name

soluble P-selectin,sP-Selectin

Abbreviation

sP-Selectin

Species

Sus scrofa (Pig)

Sample Types

serum, plasma, cell culture supernates, tissue homogenates

Detection Range

12.5 ng/mL-800 ng/mL

Sensitivity

3.12 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Others

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of pig sP-Selectin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	85-94
1:2	Average %	95
	Range %	92-98
1:4	Average %	87
	Range %	82-90
1:8	Average %	93
	Range %	90-96

Recovery

The recovery of pig sP-Selectin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	87	83-90
EDTA plasma (n=4)	85	82-89

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
800	1.828	1.968	1.898	1.750
400	1.179	1.236	1.208	1.060
200	0.734	0.757	0.746	0.598
100	0.485	0.476	0.481	0.333
50	0.311	0.323	0.317	0.169
25	0.251	0.257	0.254	0.106
12.5	0.188	0.182	0.185	0.037
0	0.145	0.151	0.148

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This Pig sP-Selectin ELISA Kit was designed for the quantitative measurement of Pig sP-Selectin protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 12.5 ng/mL-800 ng/mL and the sensitivity is 3.12 ng/mL.

Hypothermic effects on gas exchange performance of membrane oxygenator and blood coagulation during cardiopulmonary bypass in pigs K Akeho,Perfusion,2020

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.