Pig soluble P-selectin,sP-Selectin ELISA Kit

Code CSB-E08106p
Size 96T,5×96T,10×96T How to order?
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Pig sP-Selectin ELISA Kit was designed for the quantitative measurement of Pig sP-Selectin protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 12.5 ng/mL-800 ng/mL and the sensitivity is 3.12 ng/mL.

Target Name soluble P-selectin,sP-Selectin
Abbreviation sP-Selectin
Species Sus scrofa (Pig)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 12.5 ng/mL-800 ng/mL
Sensitivity 3.12 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of pig sP-Selectin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %89
Range %85-94
1:2Average %95
Range %92-98
1:4Average %87
Range %82-90
1:8Average %93
Range %90-96
Recovery
The recovery of pig sP-Selectin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8783-90
EDTA plasma (n=4)8582-89
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
8001.828 1.968 1.898 1.750
4001.179 1.236 1.208 1.060
2000.734 0.757 0.746 0.598
1000.485 0.476 0.481 0.333
500.311 0.323 0.317 0.169
250.251 0.257 0.254 0.106
12.50.188 0.182 0.185 0.037
00.145 0.151 0.148
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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