Rabbit soluble P-selectin,sP-selectin ELISA Kit

Instructions
Code CSB-E06861Rb
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name soluble P-selectin,sP-selectin
Abbreviation sP-selectin
Species Oryctolagus cuniculus (Rabbit)
Sample Types serum, plasma, cell culture supernates, urine, saliva, tissue homogenates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.8 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rabbit sP-selectin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:20Average %98
Range %95-101
1:40Average %96
Range %93-99
1:80Average %87
Range %85-90
1:160Average %92
Range %87-95
Recovery
The recovery of rabbit sP-selectin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9693-99
EDTA plasma (n=4)10199-104
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2Average
20000.090 0.092 0.091
10000.135 0.126 0.131
5000.209 0.204 0.207
2500.319 0.317 0.318
1250.457 0.446 0.452
62.50.581 0.598 0.590
31.250.812 0.836 0.824
01.571 1.598 1.585
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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