Rabbit soluble P-selectin,sP-selectin ELISA Kit

Code CSB-E06861Rb
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
soluble P-selectin,sP-selectin
Abbreviation
sP-selectin
Species
Oryctolagus cuniculus (Rabbit)
Sample Types
serum, plasma, cell culture supernates, urine, saliva, tissue homogenates
Detection Range
31.25 pg/mL-2000 pg/mL
Sensitivity
7.8 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Others
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rabbit sP-selectin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:20Average %98
Range %95-101
1:40Average %96
Range %93-99
1:80Average %87
Range %85-90
1:160Average %92
Range %87-95
Recovery
The recovery of rabbit sP-selectin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9693-99
EDTA plasma (n=4)10199-104
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2Average
20000.090 0.092 0.091
10000.135 0.126 0.131
5000.209 0.204 0.207
2500.319 0.317 0.318
1250.457 0.446 0.452
62.50.581 0.598 0.590
31.250.812 0.836 0.824
01.571 1.598 1.585
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Rabbit sP-selectin ELISA Kit was designed for the quantitative measurement of Rabbit sP-selectin protein in serum, plasma, cell culture supernates, urine, saliva, tissue homogenates. It is a Competitive ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL .

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