Rabbit soluble P-selectin,sP-selectin ELISA Kit

Rabbit soluble P-selectin,sP-selectin ELISA Kit

Product Details

Citations

Customer Reviews and Q&A

Product Details

Citations

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Customer Reviews and Q&A

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Rabbit soluble P-selectin,sP-selectin ELISA Kit

Citations (1) Protocol

Code	CSB-E06861Rb
Size	96T,5×96T,10×96T
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Product Details
Citations (1)
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Target Name

soluble P-selectin,sP-selectin

Abbreviation

sP-selectin

Species

Oryctolagus cuniculus (Rabbit)

Sample Types

serum, plasma, cell culture supernates, urine, saliva, tissue homogenates

Detection Range

31.25 pg/mL-2000 pg/mL

Sensitivity

7.8 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Others

Assay Principle

quantitative

Measurement

Competitive

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit sP-selectin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:20	Average %	98
	Range %	95-101
1:40	Average %	96
	Range %	93-99
1:80	Average %	87
	Range %	85-90
1:160	Average %	92
	Range %	87-95

Recovery

The recovery of rabbit sP-selectin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	93-99
EDTA plasma (n=4)	101	99-104

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
2000	0.090	0.092	0.091
1000	0.135	0.126	0.131
500	0.209	0.204	0.207
250	0.319	0.317	0.318
125	0.457	0.446	0.452
62.5	0.581	0.598	0.590
31.25	0.812	0.836	0.824
0	1.571	1.598	1.585

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This Rabbit sP-selectin ELISA Kit was designed for the quantitative measurement of Rabbit sP-selectin protein in serum, plasma, cell culture supernates, urine, saliva, tissue homogenates. It is a Competitive ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL .

Dyslipidemia induced inflammatory status, platelet activation and endothelial dysfunction in rabbits: Protective role of 10-Dehydrogingerdione Mohamed MahmoudEl-Seweidy, et al,Biomedicine & Pharmacotherapy ,2018

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.