Rabbit Coagulation factor VII(F7) ELISA kit

Instructions
Code CSB-EL007930RB
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name coagulation factor VII (serum prothrombin conversion accelerator)
Alternative Names F7Coagulation factor VII ELISA Kit; EC 3.4.21.21 ELISA Kit; Serum prothrombin conversion accelerator) [Cleaved into: Factor VII light chain; Factor VII heavy chain] ELISA Kit
Abbreviation F7
Uniprot No. P98139
Species Oryctolagus cuniculus (Rabbit)
Sample Types serum, plasma, tissue homogenates
Detection Range 3.9 ng/mL-250 ng/mL
Sensitivity 1 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Blood Coagulation
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rabbit F7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:20 Average % 85
Range % 80-90
1:40 Average % 104
Range % 100-107
1:80 Average % 87
Range % 83-90
1:160 Average % 102
Range % 98-106
Recovery
The recovery of rabbit F7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 88 84-93
EDTA plasma (n=4) 110 107-113
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average
250 0.134 0.130 0.132
125 0.219 0.221 0.220
62.5 0.378 0.381 0.380
31.2 0.565 0.581 0.573
15.6 0.868 0.858 0.863
7.8 1.209 1.236 1.223
3.9 1.598 1.569 1.584
0 2.399 2.401 2.400
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

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Target Background

Function
(From Uniprot)
Initiates the extrinsic pathway of blood coagulation. Serine protease that circulates in the blood in a zymogen form. Factor VII is converted to factor VIIa by factor Xa, factor XIIa, factor IXa, or thrombin by minor proteolysis. In the presence of tissue factor and calcium ions, factor VIIa then converts factor X to factor Xa by limited proteolysis. Factor VIIa will also convert factor IX to factor IXa in the presence of tissue factor and calcium (By similarity).
Gene References into Functions
  1. Results suggest that inhibition of FVIIa with small-molecule active-site inhibitors represents a promising antithrombotic approach. PMID: 20589312
Subcellular Location Secreted
Protein Families Peptidase S1 family
Tissue Specificity Plasma.
Database Links

KEGG: ocu:100009399

STRING: 9986.ENSOCUP00000024648

UniGene: Ocu.2617

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