Rabbit Complement 3,C3 ELISA Kit

Code CSB-E08668Rb
Size 96T,5×96T,10×96T
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
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Product Details

Target Name
complement component 3
Alternative Names
AHUS5, ARMD9, ASP, CPAMD1, acylation-stimulating protein cleavage product|complement component C3
Abbreviation
C3
Species
Oryctolagus cuniculus (Rabbit)
Sample Types
serum, plasma
Detection Range
27.3 ng/mL-7000 ng/mL
Sensitivity
27.3 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rabbit C3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1000Average %97
Range %91-110
1:2000Average %89
Range %82-99
1:4000Average %95
Range %85-99
1:8000Average %91
Range %87-98
Recovery
The recovery of rabbit C3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9382-97
EDTA plasma (n=4)9787-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2Average
70000.196 0.189 0.193
17500.378 0.372 0.375
437.50.812 0.805 0.809
109.41.492 1.501 1.497
27.32.012 2.003 2.008
02.913 2.897 2.905
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days
Description

This Rabbit Complement 3 (C3) ELISA Kit was designed for the quantitative measurement of Rabbit Complement 3 (C3) protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 27.3 ng/mL-7000 ng/mL and the sensitivity is 27.3 ng/mL.

Citations

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 Q&A
Q:

What is the standard in the Rabbit Complement 3 ELISA kit?
code CSB-E08668Rb. I want to know if it is actually rabbit complement C3. If you could get me a molecular weight of the standard, that will be helpful. I need to be able to convert between mg/ml and molarity for my assay and to do that I need the molecular weight of the protein. NO sequence needed, just a weight in kD.

A:
Thanks for your inquiry.
CSB-E08668Rb
Standard is purified crude extract from native samples.
We don't suggest you convert the unit. Pls kindly follow the unit in the manual.
If you want to convert it, you can refer to: https://www.uniprot.org/uniprot/P12247.
Pls let me know if you have any further questions. Thank you.
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