Rabbit Interferon γ,IFN-γ ELISA Kit

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Rabbit Interferon γ,IFN-γ ELISA Kit

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Protocol

Code	CSB-E06925Rb-IS
Size	96T,5×96T,10×96T
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Product Details
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Customer Reviews and Q&A
Target Background

Target Name

interferon, gamma

Associated names

IFNG ELISA Kit; Interferon gamma ELISA Kit; IFN-gamma ELISA Kit

Abbreviation

IFNG

Uniprot No.

P30123

Species

Oryctolagus cuniculus (Rabbit)

Sample Types

serum, plasma, tissue homogenates

Detection Range

15.6pg/ml-1000pg/ml

Sensitivity

7.8pg/ml

Assay Time

3.5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IFN-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	95
	Range %	88-100
1:2	Average %	100
	Range %	91-105
1:4	Average %	90
	Range %	84-94
1:8	Average %	113
	Range %	106-118

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.640	2.748	2.694	2.639
500	1.771	1.681	1.726	1.671
250	1.026	0.996	1.011	0.956
125	0.634	0.623	0.628	0.574
62.5	0.313	0.319	0.316	0.261
31.2	0.204	0.213	0.208	0.154
15.6	0.132	0.126	0.129	0.074
0	0.056	0.053	0.055

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function
(From Uniprot)

Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation. Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation. Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription. Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits. In turn, increases the quantity, quality, and repertoire of peptides for class I MHC loading. Increases the efficiency of peptide generation also by inducing the expression of activator PA28 that associates with the proteasome and alters its proteolytic cleavage preference. Up-regulates as well MHC II complexes on the cell surface by promoting expression of several key molecules such as cathepsins B/CTSB, H/CTSH, and L/CTSL. Participates in the regulation of hematopoietic stem cells during development and under homeostatic conditions by affecting their development, quiescence, and differentiation.

Subcellular Location

Secreted.

Protein Families

Type II (or gamma) interferon family

Tissue Specificity

Released primarily from activated T lymphocytes.

Database Links

KEGG: ocu:100008602

STRING: 9986.ENSOCUP00000021866

UniGene: Ocu.2149

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Related pathways

HIF-1 signaling pathway Jak-STAT signaling pathway TGF-beta signaling pathway Necroptosis Antigen processing and presentation IL-17 signaling pathway Natural killer cell mediated cytotoxicity Osteoclast differentiation T cell receptor signaling pathway Th1 and Th2 cell differentiation Th17 cell differentiation

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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