Rabbit Osteocalcin/bone gla protein,OT/BGP ELISA Kit

Instructions
Code CSB-E14060Rb
Size 96T,5×96T,10×96T

Product Details

Target Name bone gamma-carboxyglutamate (gla) protein
Alternative Names RP11-54H19.5, BGP, OC, PMF1, bone Gla protein|bone gamma-carboxyglutamate (gla) protein (osteocalcin)|gamma-carboxyglutamic acid-containing protein|osteocalcin
Abbreviation BGLAP
Uniprot No. P39056
Species Oryctolagus cuniculus (Rabbit)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 1.25 ng/mL-80 ng/mL
Sensitivity 0.31 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rabbit OT/BGP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 87  
Range % 82-93  
1:2 Average % 95  
Range % 89-99  
1:4 Average % 94  
Range % 90-90  
1:8 Average % 94  
Range % 86-98  
Recovery
The recovery of rabbit OT/BGP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 92 90-94  
EDTA plasma (n=4) 92 87-96  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
80 2.780 2.701 2.741 2.641  
40 2.124 2.056 2.090 1.990  
20 1.266 1.234 1.250 1.150  
10 0.667 0.621 0.644 0.544  
5 0.353 0.335 0.344 0.244  
2.5 0.262 0.249 0.256 0.156  
1.25 0.219 0.208 0.214 0.114  
0 0.102 0.098 0.100    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
Still Have Questions? Leave a Message or Start an on-line Chat

Target Data

Function Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium.
Subcellular Location Secreted
Protein Families Osteocalcin/matrix Gla protein family
Database Links

STRING: 9986.ENSOCUP00000020318

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