Rabbit Tartrate-resistant acid phosphatase 5b (TRAP5b) ELISA Kit

Instructions
Code CSB-E15043Rb
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name Tartrate-resistant acid phosphatase 5b (TRAP5b)
Abbreviation TRAP5b
Uniprot No. O97860
Species Oryctolagus cuniculus (Rabbit)
Sample Types serum, plasma, tissue homogenates
Detection Range 78 μU/mL-5000 μU/mL
Sensitivity 19.5 μU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rabbit TRAP5b in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %97
Range %88-101
1:2Average %88
Range %81-94
1:4Average %97
Range %87-101
1:8Average %91
Range %84-95
Recovery
The recovery of rabbit TRAP5b spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9084-96
EDTA plasma (n=4)10393-107
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
μU/mlOD1OD2AverageCorrected
50001.994 1.912 1.953 1.835
25001.776 1.745 1.761 1.643
12501.504 1.561 1.533 1.415
6251.212 1.178 1.195 1.077
3120.929 0.900 0.915 0.797
1560.506 0.498 0.502 0.384
780.289 0.282 0.286 0.168
00.116 0.120 0.118
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
Still Have Questions? Leave a Message or Start an on-line Chat

Target Data

Subcellular Location Lysosome
Database Links

KEGG: ocu:100008599

UniGene: Ocu.1775

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