Rabbit apolipoprotein A1 (Apo-A1) ELISA Kit

Instructions
Code CSB-E09804Rb
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name apolipoprotein A-I
Alternative Names APOA1Apolipoprotein A-I ELISA kit; Apo-AI ELISA kit; ApoA-I ELISA kit; Apolipoprotein A1) [Cleaved into: Proapolipoprotein A-I ELISA kit; ProapoA-I); Truncated apolipoprotein A-I] ELISA kit
Abbreviation APOA1
Uniprot No. P09809
Species Oryctolagus cuniculus (Rabbit)
Sample Types serum, plasma, tissue homogenates
Detection Range 6.25 μg/mL-400 μg/mL
Sensitivity 1.56 μg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rabbit Apo-A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 84  
Range % 80-92  
1:2 Average % 99  
Range % 91-105  
1:4 Average % 89  
Range % 80-96  
1:8 Average % 94  
Range % 85-99  
Recovery
The recovery of rabbit Apo-A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 93 89-98  
EDTA plasma (n=4) 98 89-107  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
µg/ml OD1 OD2 Average Corrected  
400 2.748 2.712 2.730 2.645  
200 2.234 2.222 2.228 2.143  
100 1.517 1.446 1.482 1.397  
50 0.875 0.839 0.857 0.772  
25 0.433 0.424 0.429 0.344  
12.5 0.212 0.206 0.209 0.124  
6.25 0.141 0.138 0.140 0.055  
0 0.087 0.083 0.085    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Target Data

Function Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.
Gene References into Functions
  1. Local HDAdApoAI vascular gene therapy may promote rapid regression of small-to-moderate-sized atherosclerotic lesions. PMID: 27932352
  2. These differentially expressed proteins associated with key mechanisms involved in atherosclerosis and signaling mechanisms related with vitamin E. PMID: 23796494
  3. This study showed that apoA-I exerted protective effects against fatty liver disease in rabbits induced by a high-fat diet, possibly through its antioxidant actions. PMID: 23174676
  4. The molar ratio ApoE/ApoA-I is negatively correlated with the enzyme activity, and positively correlated with increases in the intima-media thickness of common carotid wall and cardiac dysfunction signs. PMID: 22562159
Subcellular Location Secreted
Protein Families Apolipoprotein A1/A4/E family
Tissue Specificity Major protein of plasma HDL, also found in chylomicrons.
Database Links

KEGG: ocu:100009253

STRING: 9986.ENSOCUP00000009241

UniGene: Ocu.1800

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