Rabbit interleukin 12 (IL-12/P70) ELISA kit

Rabbit interleukin 12 (IL-12/P70) ELISA kit

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Rabbit interleukin 12 (IL-12/P70) ELISA kit

Name: Rabbit interleukin 12 (IL-12/P70) ELISA kit
Brand: CUSABIO
SKU: CSB-EQ027628RB

^Instructions

Code	CSB-EQ027628RB
Size	96T,5×96T,10×96T
Trial Size
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Target Name

interleukin 12 (IL-12/P70)

Alternative Names

IL12/P70, Interleukin-12/P70

Abbreviation

IL12/P70

Species

Oryctolagus cuniculus (Rabbit)

Sample Types

serum, plasma, tissue homogenates

Detection Range

3.12 pg/mL-200 pg/mL

Sensitivity

0.78 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IL-12 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	89-95
1:2	Average %	90
	Range %	83-99
1:4	Average %	95
	Range %	92-102
1:8	Average %	93
	Range %	86-99

Recovery

The recovery of rabbit IL-12 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	86-110
EDTA plasma (n=4)	97	89-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
200	2.096	2.108	2.102	1.938
100	1.678	1.651	1.665	1.501
50	1.254	1.237	1.246	1.082
25	0.896	0.905	0.901	0.737
12.5	0.614	0.642	0.628	0.464
6.25	0.378	0.364	0.371	0.207
3.12	0.252	0.249	0.251	0.087
0	0.161	0.166	0.164

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.