Rabbit interleukin 12 (IL-12/P70) ELISA kit

Code CSB-EQ027628RB
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name interleukin 12 (IL-12/P70)
Alternative Names IL12/P70, Interleukin-12/P70
Abbreviation IL12/P70
Species Oryctolagus cuniculus (Rabbit)
Sample Types serum, plasma, tissue homogenates
Detection Range 3.12 pg/mL-200 pg/mL
Sensitivity 0.78 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IL-12 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:1Average %93
Range %89-95
1:2Average %90
Range %83-99
1:4Average %95
Range %92-102
1:8Average %93
Range %86-99
The recovery of rabbit IL-12 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9586-110
EDTA plasma (n=4)9789-99
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
2002.096 2.108 2.102 1.938
1001.678 1.651 1.665 1.501
501.254 1.237 1.246 1.082
250.896 0.905 0.901 0.737
12.50.614 0.642 0.628 0.464
6.250.378 0.364 0.371 0.207
3.120.252 0.249 0.251 0.087
00.161 0.166 0.164
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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