Target Name |
interleukin 12 (IL-12/P70) |
Alternative Names |
IL12/P70, Interleukin-12/P70 |
Abbreviation |
IL12/P70 |
Species |
Oryctolagus cuniculus (Rabbit) |
Sample Types |
serum, plasma, tissue homogenates |
Detection Range |
3.12 pg/mL-200 pg/mL |
Sensitivity |
0.78 pg/mL |
Assay Time |
1-5h |
Sample Volume |
50-100ul |
Detection Wavelength |
450 nm |
Research Area |
Immunology
|
Assay Principle |
quantitative |
Measurement |
Sandwich |
Precision |
Intra-assay Precision (Precision within an assay): CV%<8% | | | | Three samples of known concentration were tested twenty times on one plate to assess. | | Inter-assay Precision (Precision between assays): CV%<10% | | | | Three samples of known concentration were tested in twenty assays to assess. | | | | | | | | | |
|
Linearity |
To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IL-12 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | | Sample | Serum(n=4) | | 1:1 | Average % | 93 | | Range % | 89-95 | | 1:2 | Average % | 90 | | Range % | 83-99 | | 1:4 | Average % | 95 | | Range % | 92-102 | | 1:8 | Average % | 93 | | Range % | 86-99 | |
|
Recovery |
The recovery of rabbit IL-12 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
Sample Type | Average % Recovery | Range | | Serum (n=5) | 95 | 86-110 | | EDTA plasma (n=4) | 97 | 89-99 | | | | | | | | | | | | | | | |
|
Typical Data |
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
pg/ml | OD1 | OD2 | Average | Corrected | | 200 | 2.096 | 2.108 | 2.102 | 1.938 | | 100 | 1.678 | 1.651 | 1.665 | 1.501 | | 50 | 1.254 | 1.237 | 1.246 | 1.082 | | 25 | 0.896 | 0.905 | 0.901 | 0.737 | | 12.5 | 0.614 | 0.642 | 0.628 | 0.464 | | 6.25 | 0.378 | 0.364 | 0.371 | 0.207 | | 3.12 | 0.252 | 0.249 | 0.251 | 0.087 | | 0 | 0.161 | 0.166 | 0.164 | | |
|
|
Troubleshooting
and FAQs |
ELISA kit FAQs |
Storage |
Store at 2-8°C. Please refer to protocol. |
Lead Time |
3-5 working days |