Dog interleukin 12 (IL-12/P70) ELISA kit

Dog interleukin 12 (IL-12/P70) ELISA kit

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ELISA Kit
Dog interleukin 12 (IL-12/P70) ELISA kit

Protocol

Code	CSB-EQ027628DO
Size	96T,5×96T,10×96T
Trial Size
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Description

This Dog IL12/P70 ELISA Kit was designed for the quantitative measurement of Dog IL12/P70 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL .

Target Name

interleukin 12 (IL-12/P70)

Alternative Names

IL12/P70, Interleukin-12/P70

Abbreviation

IL12/P70

Species

Canis lupus familiaris (Dog) (Canis familiaris)

Sample Types

serum, plasma, cell culture supernates, tissue homogenates

Detection Range

15.6 pg/mL-1000 pg/mL

Sensitivity

3.9 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of dog IL-12/P70 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	91-105
1:2	Average %	87
	Range %	84-93
1:4	Average %	97
	Range %	91-106
1:8	Average %	94
	Range %	86-98

Recovery

The recovery of dog IL-12/P70 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	87-96
EDTA plasma (n=4)	97	92-106

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.912	2.945	2.929	2.829
500	2.546	2.578	2.562	2.462
250	1.780	1.806	1.793	1.693
125	1.127	1.226	1.177	1.077
62.5	0.546	0.576	0.561	0.461
31.2	0.259	0.269	0.264	0.164
15.6	0.168	0.176	0.172	0.072
0	0.095	0.105	0.100

ELISA Data Analysis

Watch ELISA data processing video & download Curve Expert if needed

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

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Check Code：

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.