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Dog interleukin 12 (IL-12/P70) ELISA kit

Instructions
Code CSB-EQ027628DO
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name interleukin 12 (IL-12/P70)
Alternative Names IL12/P70, Interleukin-12/P70
Abbreviation IL12/P70
Species Canis lupus familiaris (Dog) (Canis familiaris)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of dog IL-12/P70 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　SampleSerum(n=4)
1:1Average %95
Range %91-105
1:2Average %87
Range %84-93
1:4Average %97
Range %91-106
1:8Average %94
Range %86-98
Recovery
The recovery of dog IL-12/P70 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9087-96
EDTA plasma (n=4)9792-106
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
10002.912 2.945 2.929 2.829
5002.546 2.578 2.562 2.462
2501.780 1.806 1.793 1.693
1251.127 1.226 1.177 1.077
62.50.546 0.576 0.561 0.461
31.20.259 0.269 0.264 0.164
15.60.168 0.176 0.172 0.072
00.095 0.105 0.100 　
Troubleshooting
and FAQs		 ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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