| Description |
This Dog IL12/P70 ELISA Kit was designed for the quantitative measurement of Dog IL12/P70 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL . |
| Target Name |
interleukin 12 (IL-12/P70) |
| Alternative Names |
IL12/P70, Interleukin-12/P70 |
| Abbreviation |
IL12/P70 |
| Species |
Canis lupus familiaris (Dog) (Canis familiaris) |
| Sample Types |
serum, plasma, cell culture supernates, tissue homogenates |
| Detection Range |
15.6 pg/mL-1000 pg/mL |
| Sensitivity |
3.9 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology
|
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | | Three samples of known concentration were tested twenty times on one plate to assess. | | | Inter-assay Precision (Precision between assays): CV%<10% | | | | | Three samples of known concentration were tested in twenty assays to assess. | | | | | | | | | | |
|
| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of dog IL-12/P70 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | | | Sample | Serum(n=4) | | | 1:1 | Average % | 95 | | | Range % | 91-105 | | | 1:2 | Average % | 87 | | | Range % | 84-93 | | | 1:4 | Average % | 97 | | | Range % | 91-106 | | | 1:8 | Average % | 94 | | | Range % | 86-98 | |
|
| Recovery |
| The recovery of dog IL-12/P70 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
| Sample Type | Average % Recovery | Range | | | Serum (n=5) | 90 | 87-96 | | | EDTA plasma (n=4) | 97 | 92-106 | | | | | | | | | | | | | | | | | |
|
| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
 | pg/ml | OD1 | OD2 | Average | Corrected | | | 1000 | 2.912 | 2.945 | 2.929 | 2.829 | | | 500 | 2.546 | 2.578 | 2.562 | 2.462 | | | 250 | 1.780 | 1.806 | 1.793 | 1.693 | | | 125 | 1.127 | 1.226 | 1.177 | 1.077 | | | 62.5 | 0.546 | 0.576 | 0.561 | 0.461 | | | 31.2 | 0.259 | 0.269 | 0.264 | 0.164 | | | 15.6 | 0.168 | 0.176 | 0.172 | 0.072 | | | 0 | 0.095 | 0.105 | 0.100 | | |
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| ELISA Data Analysis |
Watch ELISA data processing video & download Curve Expert if needed |
Troubleshooting
and FAQs |
ELISA kit FAQs |
| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
