Target Name
matrix metallopeptidase 1 (interstitial collagenase)
Alternative Names
MMP1Interstitial collagenase ELISA Kit; EC 3.4.24.7 ELISA Kit; Matrix metalloproteinase-1 ELISA Kit; MMP-1 ELISA Kit
Species
Oryctolagus cuniculus (Rabbit)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.156 ng/mL-10 ng/mL
Detection Wavelength
450 nm
Assay Principle
quantitative
Precision
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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Linearity
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit MMP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
95 |
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| Range % |
90-100 |
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| 1:2 |
Average % |
104 |
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| Range % |
97-109 |
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| 1:4 |
Average % |
88 |
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| Range % |
82-92 |
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| 1:8 |
Average % |
98 |
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| Range % |
91-102 |
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Recovery
| The recovery of rabbit MMP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
88 |
82-92 |
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| EDTA plasma (n=4) |
102 |
92-107 |
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Typical Data
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 10 |
2.590 |
2.545 |
2.568 |
2.466 |
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| 5 |
1.963 |
1.922 |
1.943 |
1.841 |
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| 2.5 |
1.435 |
1.400 |
1.418 |
1.316 |
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| 1.25 |
0.841 |
0.818 |
0.830 |
0.728 |
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| 0.625 |
0.448 |
0.427 |
0.438 |
0.336 |
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| 0.312 |
0.328 |
0.317 |
0.323 |
0.221 |
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| 0.156 |
0.248 |
0.242 |
0.245 |
0.143 |
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| 0 |
0.101 |
0.103 |
0.102 |
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Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description
This Rabbit MMP1 ELISA Kit was designed for the quantitative measurement of Rabbit MMP1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.
