Rat 3-oxo-5-alpha-steroid 4-dehydrogenase 2(SRD5A2) ELISA kit

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Description

This Rat SRD5A2 ELISA Kit was designed for the quantitative measurement of Rat SRD5A2 protein in serum, plasma, tissue homogenates, cell lysates. It is a Competitive ELISA kit, its detection range is 6 pg/mL-1200 pg/mL and the sensitivity is 3 pg/mL.

Target Name

steroid-5-alpha-reductase, alpha polypeptide 2 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 2)

Alternative Names

Srd5a2; 3-oxo-5-alpha-steroid 4-dehydrogenase 2; 5 alpha-SR2; SR type 2; Steroid 5-alpha-reductase 2; S5AR 2

Abbreviation

SRD5A2

Uniprot No.

P31214

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, tissue homogenates, cell lysates

Detection Range

6 pg/mL-1200 pg/mL

Sensitivity

3 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Competitive

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat SRD5A2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	88-96
1:2	Average %	94
	Range %	89-99
1:4	Average %	96
	Range %	90-103
1:8	Average %	90
	Range %	87-94

Recovery

The recovery of rat SRD5A2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	95-101
EDTA plasma (n=4)	93	87-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
1200	0.225	0.217	0.221
300	0.411	0.434	0.423
96	0.651	0.632	0.642
24	1.055	1.017	1.036
6	1.497	1.458	1.478
0	2.359	2.450	2.405

ELISA Data Analysis

Watch ELISA data processing video & download Curve Expert if needed

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Target Background

Function (From Uniprot)	Converts testosterone (T) into 5-alpha-dihydrotestosterone (DHT) and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology.
Gene References into Functions	Results suggest that in the acute phase after spinal cord injury, the increased expression of 18kDa translocator protein and 5alpha-reductase may represent a protective endogenous response against tissue injury. PMID: 27150077 findings show that, in the adult brain, 5alphaR2 is distributed in critical regions for behavioral regulation, suggesting that the functional role of this isoform is present throughout the entire lifespan of the individual PMID: 22776423 Low Srd5a2 expression is associated with benign prostatic hypertrophy. PMID: 23405234 Changes in the expression of steroid 5-alpha reductase isoenzymes in the prostate in response to environmental stress. PMID: 23122426 Tesosterone concentrations during postnatal sexual differentiation of the rat central nervous system, among other sex-dependent factors, influence brain levels of 5alpha-Reductase isozymes in adulthood PMID: 22131296 5-alpha-reductase type 2 is confined to motoneuronal cells of anterior horns of rat spinal cord, cells that also are known to express high levels of androgen receptor. PMID: 12899683 A method for the quantitation of mRNA levels of Srd5a2 isoenzymes has been developed. PMID: 14592534 5 alpha-R2 labeling was observed in dorsal (DH) and ventral horns of gray matter (Gm). and 60% of 5 alpha-R2 immunostaining colocalized with oligodendrocyte, 25% with neuron, and 15% with astrocyte markers. PMID: 15305365 regulatory elements and the minimal promoter were mapped to the 485-bp region upstream of the start codon PMID: 15576829 A high-dietary fat and caloric intake upregulates prostate 5alpha-reductase-2 gene expression, and stimulates prostate growth in neonatal, but not adult r PMID: 16425201 great sexual dimorphism in the central nervous system (CNS) with respect to both 5alpha-reductase isozymes, and suggest a crucial role of dihydrotestosterone in the sexual dimorphism of the CNS in the female PMID: 16595696 The SRD5A2 mRNA expression in normal rat is 4-fold higher than human prostatic tissue. PMID: 16818707 Show More Hide All
Subcellular Location	Microsome membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein.
Protein Families	Steroid 5-alpha reductase family
Tissue Specificity	Expressed in high levels in the prostate and many other androgen-sensitive tissues.
Database Links	KEGG: rno:64677 STRING: 10116.ENSRNOP00000009254 UniGene: Rn.9938

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Code	CSB-EL022654RA
Size	96T,5×96T,10×96T How to order?
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Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.