Rat 3-oxo-5-alpha-steroid 4-dehydrogenase 2(SRD5A2) ELISA kit

Instructions
Code CSB-EL022654RA
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name steroid-5-alpha-reductase, alpha polypeptide 2 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 2)
Alternative Names Srd5a2 ELISA Kit; 3-oxo-5-alpha-steroid 4-dehydrogenase 2 ELISA Kit; EC 1.3.1.22 ELISA Kit; 5 alpha-SR2 ELISA Kit; SR type 2 ELISA Kit; Steroid 5-alpha-reductase 2 ELISA Kit; S5AR 2 ELISA Kit
Abbreviation SRD5A2
Uniprot No. P31214
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 6 pg/mL-1200 pg/mL
Sensitivity 3 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat SRD5A2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 92  
Range % 88-96  
1:2 Average % 94  
Range % 89-99  
1:4 Average % 96  
Range % 90-103  
1:8 Average % 90  
Range % 87-94  
Recovery
The recovery of rat SRD5A2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 98 95-101  
EDTA plasma (n=4) 93 87-100  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average    
1200 0.225 0.217 0.221    
300 0.411 0.434 0.423    
96 0.651 0.632 0.642    
24 1.055 1.017 1.036    
6 1.497 1.458 1.478    
0 2.359 2.450 2.405    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

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Target Background

Function
(From Uniprot)
Converts testosterone (T) into 5-alpha-dihydrotestosterone (DHT) and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids
Gene References into Functions
  1. Results suggest that in the acute phase after spinal cord injury, the increased expression of 18kDa translocator protein and 5alpha-reductase may represent a protective endogenous response against tissue injury. PMID: 27150077
  2. findings show that, in the adult brain, 5alphaR2 is distributed in critical regions for behavioral regulation, suggesting that the functional role of this isoform is present throughout the entire lifespan of the individual PMID: 22776423
  3. Low Srd5a2 expression is associated with benign prostatic hypertrophy. PMID: 23405234
  4. Changes in the expression of steroid 5-alpha reductase isoenzymes in the prostate in response to environmental stress. PMID: 23122426
  5. Tesosterone concentrations during postnatal sexual differentiation of the rat central nervous system, among other sex-dependent factors, influence brain levels of 5alpha-Reductase isozymes in adulthood PMID: 22131296
  6. 5-alpha-reductase type 2 is confined to motoneuronal cells of anterior horns of rat spinal cord, cells that also are known to express high levels of androgen receptor. PMID: 12899683
  7. A method for the quantitation of mRNA levels of Srd5a2 isoenzymes has been developed. PMID: 14592534
  8. 5 alpha-R2 labeling was observed in dorsal (DH) and ventral horns of gray matter (Gm). and 60% of 5 alpha-R2 immunostaining colocalized with oligodendrocyte, 25% with neuron, and 15% with astrocyte markers. PMID: 15305365
  9. regulatory elements and the minimal promoter were mapped to the 485-bp region upstream of the start codon PMID: 15576829
  10. A high-dietary fat and caloric intake upregulates prostate 5alpha-reductase-2 gene expression, and stimulates prostate growth in neonatal, but not adult r PMID: 16425201
  11. great sexual dimorphism in the central nervous system (CNS) with respect to both 5alpha-reductase isozymes, and suggest a crucial role of dihydrotestosterone in the sexual dimorphism of the CNS in the female PMID: 16595696
  12. The SRD5A2 mRNA expression in normal rat is 4-fold higher than human prostatic tissue. PMID: 16818707

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Subcellular Location Microsome membrane, Multi-pass membrane protein, Endoplasmic reticulum membrane, Multi-pass membrane protein
Protein Families Steroid 5-alpha reductase family
Tissue Specificity Expressed in high levels in the prostate and many other androgen-sensitive tissues.
Database Links

KEGG: rno:64677

STRING: 10116.ENSRNOP00000009254

UniGene: Rn.9938

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