Rat 3-oxo-5-alpha-steroid 4-dehydrogenase 2(SRD5A2) ELISA kit

Product Details

Citations

Related Products

Customer Reviews and Q&A

Target Background

Product Details

Target Name

steroid-5-alpha-reductase, alpha polypeptide 2 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 2)

Alternative Names

Srd5a2; 3-oxo-5-alpha-steroid 4-dehydrogenase 2; 5 alpha-SR2; SR type 2; Steroid 5-alpha-reductase 2; S5AR 2

Abbreviation

SRD5A2

Uniprot No.

P31214

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, tissue homogenates, cell lysates

Detection Range

6 pg/mL-1200 pg/mL

Sensitivity

3 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Competitive

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat SRD5A2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	88-96
1:2	Average %	94
	Range %	89-99
1:4	Average %	96
	Range %	90-103
1:8	Average %	90
	Range %	87-94

Recovery

The recovery of rat SRD5A2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	95-101
EDTA plasma (n=4)	93	87-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
1200	0.225	0.217	0.221
300	0.411	0.434	0.423
96	0.651	0.632	0.642
24	1.055	1.017	1.036
6	1.497	1.458	1.478
0	2.359	2.450	2.405

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

The Rat 3-oxo-5-alpha-steroid 4-dehydrogenase 2 (SRD5A2) ELISA kit is the ultimate tool for researchers seeking to quantify SRD5A2 levels in samples of rat serum, plasma, tissue homogenates, and cell lysates.

The SRD5A2 protein, also known as 3-oxo-5-alpha-steroid 4-dehydrogenase 2, is involved in processing androgens, which are hormones that direct male sexual development,making it an essential protein for researchers in this field. Our ELISA kit is designed to provide fast, accurate, and reliable results, allowing researchers to measure SRD5A2 levels with high sensitivity and precision.

With a detection range of 6 pg/mL to 1200 pg/mL and a sensitivity of 3 pg/mL, this ELISA kit delivers precise and accurate results. The assay time is just 1-5 hours, and the sample volume required is only 50-100ul, making it a highly efficient tool for researchers.

The kit uses a competitive assay principle, enabling quantitative measurement of SRD5A2 levels in samples. The detection wavelength is 450 nm, making it easy to read and interpret results.

Citations

Target Background

Function
(From Uniprot)

Converts testosterone (T) into 5-alpha-dihydrotestosterone (DHT) and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology.

Gene References into Functions

Results suggest that in the acute phase after spinal cord injury, the increased expression of 18kDa translocator protein and 5alpha-reductase may represent a protective endogenous response against tissue injury. PMID: 27150077
findings show that, in the adult brain, 5alphaR2 is distributed in critical regions for behavioral regulation, suggesting that the functional role of this isoform is present throughout the entire lifespan of the individual PMID: 22776423
Low Srd5a2 expression is associated with benign prostatic hypertrophy. PMID: 23405234
Changes in the expression of steroid 5-alpha reductase isoenzymes in the prostate in response to environmental stress. PMID: 23122426
Tesosterone concentrations during postnatal sexual differentiation of the rat central nervous system, among other sex-dependent factors, influence brain levels of 5alpha-Reductase isozymes in adulthood PMID: 22131296
5-alpha-reductase type 2 is confined to motoneuronal cells of anterior horns of rat spinal cord, cells that also are known to express high levels of androgen receptor. PMID: 12899683
A method for the quantitation of mRNA levels of Srd5a2 isoenzymes has been developed. PMID: 14592534
5 alpha-R2 labeling was observed in dorsal (DH) and ventral horns of gray matter (Gm). and 60% of 5 alpha-R2 immunostaining colocalized with oligodendrocyte, 25% with neuron, and 15% with astrocyte markers. PMID: 15305365
regulatory elements and the minimal promoter were mapped to the 485-bp region upstream of the start codon PMID: 15576829
A high-dietary fat and caloric intake upregulates prostate 5alpha-reductase-2 gene expression, and stimulates prostate growth in neonatal, but not adult r PMID: 16425201
great sexual dimorphism in the central nervous system (CNS) with respect to both 5alpha-reductase isozymes, and suggest a crucial role of dihydrotestosterone in the sexual dimorphism of the CNS in the female PMID: 16595696
The SRD5A2 mRNA expression in normal rat is 4-fold higher than human prostatic tissue. PMID: 16818707

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Subcellular Location

Microsome membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein.

Protein Families

Steroid 5-alpha reductase family

Tissue Specificity

Expressed in high levels in the prostate and many other androgen-sensitive tissues.

Database Links

KEGG: rno:64677

STRING: 10116.ENSRNOP00000009254

UniGene: Rn.9938

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Code	CSB-EL022654RA
Size	96T,5×96T,10×96T
Price	Request a Quote
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Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.