Rat 5'-nucleotidase/cluster of differentiation 73 (NT5E/CD73) ELISA kit

Code CSB-E14100r
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name 5'-nucleotidase/cluster of differentiation 73 (NT5E/CD73)
Alternative Names Nt5e ELISA kit; Nt5 ELISA kit; Nte5'-nucleotidase ELISA kit; 5'-NT ELISA kit; EC 3.1.3.5 ELISA kit; Ecto-5'-nucleotidase ELISA kit; CD antigen CD73 ELISA kit
Abbreviation NT5E/CD73
Uniprot No. P21588
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates, cell culture supernates
Detection Range 0.625 mIU/mL-40 mIU/mL
Sensitivity 0.156 mIU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat CD73 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %90
Range %87-96
1:2Average %87
Range %82-95
1:4Average %90
Range %85-97
1:8Average %93
Range %86-101
Recovery
The recovery of rat CD73 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9590-101
EDTA plasma (n=4)9186-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
mIU/mlOD1OD2AverageCorrected
402.504 2.416 2.460 2.358
201.933 1.824 1.879 1.777
101.386 1.283 1.335 1.233
50.814 0.749 0.782 0.680
2.50.424 0.397 0.411 0.309
1.250.228 0.213 0.221 0.119
0.6250.154 0.145 0.150 0.048
00.103 0.101 0.102  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function Hydrolyzes extracellular nucleotides into membrane permeable nucleosides.
Subcellular Location Cell membrane, Lipid-anchor, GPI-anchor
Protein Families 5'-nucleotidase family
Database Links

STRING: 10116.ENSRNOP00000015057

UniGene: Rn.40132

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