Rat C-C motif chemokine 6 (CCL6) ELISA kit

Code CSB-EL027415RA
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name C-C motif chemokine 6 (CCL6)
Alternative Names Ccl6C-C motif chemokine 6 ELISA Kit; Small-inducible cytokine A6 ELISA Kit
Abbreviation CCL6
Uniprot No. Q68FP3
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of rat CCL6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 94
Range % 87-98
1:2 Average % 98
Range % 91-104
1:4 Average % 91
Range % 85-97
1:8 Average % 97
Range % 92-103
The recovery of rat CCL6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 92 87-96
EDTA plasma (n=4) 98 93-104
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
10 2.634 2.782 2.708 2.604
5 2.178 2.358 2.268 2.164
2.5 1.572 1.556 1.564 1.460
1.25 0.852 0.860 0.856 0.752
0.625 0.413 0.426 0.420 0.316
0.312 0.317 0.301 0.309 0.205
0.156 0.204 0.200 0.202 0.098
0 0.103 0.105 0.104  
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Gene References into Functions
  1. The mouse C10 analog gene in the rat, and named it as ccl6, was identified and cloned. PMID: 23212328
Subcellular Location Secreted
Protein Families Intercrine beta (chemokine CC) family
Database Links

KEGG: rno:287910

STRING: 10116.ENSRNOP00000048296

UniGene: Rn.7857


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