Calcitonin is a 32-amino acid peptide hormone primarily produced by the parafollicular cells (C cells) of the thyroid gland. This hormone plays an important role in calcium homeostasis by inhibiting osteoclast activity, thereby reducing bone resorption and lowering blood calcium levels. Calcitonin also promotes calcium excretion by the kidneys and inhibits calcium absorption in the intestines. In research settings, calcitonin serves as an important biomarker for medullary thyroid carcinoma and other neuroendocrine tumors. This makes it valuable for both diagnostic and therapeutic monitoring studies.
The Rat Calcitonin,CT ELISA Kit (CSB-E05132r) is designed for quantitative measurement of calcitonin in rat samples using a sandwich assay principle. This kit works with serum, plasma, and tissue homogenates with a detection range of 1.56 pg/mL to 100 pg/mL and sensitivity of 0.39 pg/mL. The assay requires 50-100 μL sample volume and can be completed within 1-5 hours, with detection performed at 450 nm wavelength.
Application Examples
Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section.
This ELISA kit has been used in research studying bone metabolism and endocrine regulation. Studies have examined the relationships between various hormones and bone health markers. The kit allows quantification of calcitonin in rat serum samples as part of comprehensive biochemical analyses alongside other bone formation and resorption markers.
• Bone metabolism research - Used to measure calcitonin levels alongside bone-specific alkaline phosphatase, osteocalcin, and other bone turnover markers in studies examining bone health and metabolic processes
• Endocrine profiling - Applied in hormone analysis studies that examine circadian patterns and temporal variations in hormone concentrations, particularly in conjunction with parathyroid hormone measurements
• Inflammatory biomarker studies - Used as part of multi-analyte panels that include inflammatory cytokines to assess the interplay between endocrine factors and inflammatory responses
• Calcium homeostasis research - Applied in studies examining calcium and phosphate regulation, where calcitonin measurements complement studies of mineral metabolism and related biochemical parameters
