Rat Calcitonin,CT ELISA Kit

Code CSB-E05132r
Size 96T How to order?
Trial Size 24T ELISA kits trial application
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Product Details

Description

The rat calcitonin (CT) ELISA Kit is used to quantitatively measure rat calcitonin concentrations in serum, plasma, or tissue homogenates. It performs well in important characteristics, including sensitivity and specificity. This assay is based on the sandwich ELISA mechanism and enzyme-substrate chromogenic reaction. The solution color develops proportionally to the amount of calcitonin in the sample. And the intensity of the color can be measured at 450 nm via a microplate reader.

Calcitonin is a hormone secreted by the C-cells of the thyroid gland and mainly functions to lower calcium levels in the blood. It reduces blood calcium levels by inhibiting osteoclast activity in the bones and increasing the amount of calcium excreted in the urine. This hormone also prevents the absorption of calcium from the intestine, as well as increases the calcium absorbed by the kidney. When calcium concentration in the serum elevates, calcitonin is inducibly expressed and protects against the development of hypercalcemia. Calcitonin is also stimulated by gastrointestinal hormones such as gastrin. It is also an important tumor marker of thyroid carcinoma.

Target Name calcitonin
Alternative Names CALCA Calcitonin
Abbreviation CALCA
Uniprot No. P01257
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 1.56 pg/mL-100 pg/mL
Sensitivity 0.39 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat CT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 94  
Range % 87-101  
1:2 Average % 88  
Range % 80-95  
1:4 Average % 95  
Range % 86-106  
1:8 Average % 90  
Range % 82-100  
Recovery
The recovery of rat CT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 85-99  
EDTA plasma (n=4) 94 88-101  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
\\\"\\\"
pg/ml OD1 OD2 Average Corrected  
100 2.746 2.779 2.763 2.582  
50 2.138 2.175 2.157 1.976  
25 1.395 1.453 1.424 1.243  
12.5 0.866 0.854 0.860 0.679  
6.25 0.552 0.562 0.557 0.376  
3.12 0.428 0.401 0.415 0.234  
1.56 0.283 0.298 0.291 0.110  
0 0.179 0.183 0.181    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Causes a rapid but short-lived drop in the level of calcium and phosphate in blood by promoting the incorporation of those ions in the bones.
Subcellular Location Secreted.
Protein Families Calcitonin family
Database Links

STRING: 10116.ENSRNOP00000014948

UniGene: Rn.90085

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