Rat Calcitonin,CT ELISA Kit

Instructions
Code CSB-E05132r
Size 96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name calcitonin
Alternative Names CALCA Calcitonin
Abbreviation CALCA
Uniprot No. P01257
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 1.56 pg/mL-100 pg/mL
Sensitivity 0.39 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat CT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 94  
Range % 87-101  
1:2 Average % 88  
Range % 80-95  
1:4 Average % 95  
Range % 86-106  
1:8 Average % 90  
Range % 82-100  
Recovery
The recovery of rat CT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 85-99  
EDTA plasma (n=4) 94 88-101  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
\\\"\\\"
pg/ml OD1 OD2 Average Corrected  
100 2.746 2.779 2.763 2.582  
50 2.138 2.175 2.157 1.976  
25 1.395 1.453 1.424 1.243  
12.5 0.866 0.854 0.860 0.679  
6.25 0.552 0.562 0.557 0.376  
3.12 0.428 0.401 0.415 0.234  
1.56 0.283 0.298 0.291 0.110  
0 0.179 0.183 0.181    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Target Data

Function Causes a rapid but short-lived drop in the level of calcium and phosphate in blood by promoting the incorporation of those ions in the bones.
Subcellular Location Secreted
Protein Families Calcitonin family
Database Links

STRING: 10116.ENSRNOP00000014948

UniGene: Rn.90085

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