Rat Caspase-1(CASP1) ELISA kit

Code CSB-EL004543RA
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Uniprot No. P43527
Lead Time 5-7 working days
Abbreviation CASP1
Protein Biological Process 1 Apoptosis/Autophagy
Target Name caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase)
Alias ICE, IL1BC, P45, CASP1 nirs variant 1|IL1B-convertase|caspase 1|caspase 1, apoptosis-related cysteine protease (interleukin 1, beta, convertase)|caspase-1|interleukin 1-B converting enzyme|interleuk
Species Rattus norvegicus (Rat)
Protein Biological Process 3 Apoptosis
Sample Types serum, plasma, cell lysates, cell culture supernates
Detection Range 6.25 pg/mL-400 pg/mL
Sensitivity 1.56 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat CASP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 91
Range % 88-93
1:2 Average % 95
Range % 90-102
1:4 Average % 97
Range % 94-101
1:8 Average % 94
Range % 91-98
Recovery
The recovery of rat CASP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 90 86-94
EDTA plasma (n=4) 99 93-105
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
400 2.189 2.321 2.255 2.047
200 1.729 1.754 1.742 1.534
100 1.151 1.165 1.158 0.950
50 0.755 0.748 0.752 0.544
25 0.456 0.468 0.462 0.254
12.5 0.317 0.325 0.321 0.113
6.25 0.277 0.290 0.284 0.076
0 0.201 0.215 0.208  
References
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Function Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis. Upon inflammasome activation, during DNA virus infection but not RNA virus challenge, controls antiviral immunity through the cleavage of CGAS, rendering it inactive.
Subcellular Location Cytoplasm
Protein Families Peptidase C14A family
Database Links

STRING: 10116.ENSRNOP00000009993

UniGene: Rn.37508

Pathway Lipids and Inflammation in Atherogenesis
Necroptosis
NOD-like receptor signaling pathway

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