Rat Chitinase-3-like protein 1(CHI3L1) ELISA kit

Product Details

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Customer Reviews and Q&A

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Rat Chitinase-3-like protein 1(CHI3L1) ELISA kit

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Citations (2) Protocol MSDS

Code	CSB-EL005346RA
Size	96T,5×96T,10×96T
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Product Details
Citations (2)
Related Products
Customer Reviews and Q&A
Target Background

Target Name

chitinase 3-like 1 (cartilage glycoprotein-39)

Alternative Names

Chi3l1Chitinase-3-like protein 1 ELISA kit; Cartilage glycoprotein 39 ELISA kit; CGP-39 ELISA kit; GP-39 ELISA kit

Abbreviation

CHI3L1

Uniprot No.

Q9WTV1

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, tissue homogenates

Detection Range

4.7 ng/ml - 300 ng/ml

Sensitivity

1.17 ng/ml

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat CHI3L1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	103
	Range %	99-108
1:2	Average %	98
	Range %	94-102
1:4	Average %	93
	Range %	89-97
1:8	Average %	90
	Range %	86-98

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml

OD1

OD2

Average

Corrected

300

2.486

2.358

2.422

2.241

150

2.043

1.931

1.987

1.806

1.241

1.329

1.285

1.104

37.5

0.765

0.803

0.784

0.603

18.75

0.413

0.427

0.420

0.239

9.4

0.339

0.345

0.342

0.161

4.7

0.231

0.224

0.228

0.047

0.180

0.182

0.181

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

The Effects of Ferulic Acid Against Oxidative Stress and Inflammation in Formaldehyde-Induced Hepatotoxicity. Gerin F.et al,Inflammation.,2016
The Effects of Quercetin on Acute Lung Injury and Biomarkers of Inflammation and Oxidative Stress in the Rat Model ofSepsis Gerin F. et al,Inflammation,2015

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function
(From Uniprot)

Carbohydrate-binding lectin with a preference for chitin. Has no chitinase activity. May play a role in tissue remodeling and in the capacity of cells to respond to and cope with changes in their environment. Plays a role in T-helper cell type 2 (Th2) inflammatory response and IL-13-induced inflammation, regulating allergen sensitization, inflammatory cell apoptosis, dendritic cell accumulation and M2 macrophage differentiation. Facilitates invasion of pathogenic enteric bacteria into colonic mucosa and lymphoid organs. Mediates activation of AKT1 signaling pathway and subsequent IL8 production in colonic epithelial cells. Regulates antibacterial responses in lung by contributing to macrophage bacterial killing, controlling bacterial dissemination and augmenting host tolerance. Also regulates hyperoxia-induced injury, inflammation and epithelial apoptosis in lung.

Gene References into Functions

Results showed that protein and mRNA levels of CHI3L1 decreased in normal cartilage but increased in osteoarthritic cartilage. PMID: 26978347
NGF-treated tissue showed increased expression of CHI3L1 which was inhibited by the presence of Ro 08-2750 in intervertebral disk cells. PMID: 25062286
Data show that hepatic CD14+ cells are an YKL-40(Chi3L1)mRNA and protein source in acute and chronic liver injury, with expression patterns similar to growth factors implicated in inflammation-fibrogenesis PMID: 21987626

Subcellular Location

Secreted, extracellular space. Cytoplasm. Cytoplasm, perinuclear region. Endoplasmic reticulum.

Protein Families

Glycosyl hydrolase 18 family

Database Links

KEGG: rno:89824

STRING: 10116.ENSRNOP00000065787

UniGene: Rn.68940

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Related pathways

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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