Rat Cholecystokinin,CCK ELISA Kit

Instructions
Code CSB-E08114r
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name cholecystokinin
Alternative Names CckCholecystokinin ELISA kit; CCK) [Cleaved into: Cholecystokinin-39 ELISA kit; CCK39); Cholecystokinin-33 ELISA kit; CCK33); Cholecystokinin-22 ELISA kit; CCK22); Cholecystokinin-12 ELISA kit; CCK12); Cholecystokinin-8 ELISA kit; CCK8)] ELISA kit
Abbreviation CCK
Uniprot No. P01355
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat CCK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 93  
Range % 88-97  
1:2 Average % 95  
Range % 90-99  
1:4 Average % 97  
Range % 93-101  
1:8 Average % 84  
Range % 80-92  
Recovery
The recovery of rat CCK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 103 96-108  
EDTA plasma (n=4) 90 85-94  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
1000 2.449 2.351 2.400 2.209  
500 1.783 1.692 1.738 1.547  
250 1.212 1.182 1.197 1.006  
125 0.789 0.739 0.764 0.573  
62.5 0.549 0.596 0.573 0.382  
31.2 0.409 0.420 0.415 0.224  
15.6 0.267 0.278 0.273 0.082  
0 0.185 0.197 0.191    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function This peptide hormone induces gall bladder contraction and the release of pancreatic enzymes in the gut. Its function in the brain is not clear. Binding to CCK-A receptors stimulates amylase release from the pancreas, binding to CCK-B receptors stimulates gastric acid secretion.
Gene References into Functions
  1. Study provides the first direct comparative and quantitative data showing that presynaptic GABAB receptors exert differential inhibition at cholecystokinin (CCK) and parvalbumin (PV) basket cell (BC) synapses, resulting from a higher surface expression of the receptor at all CCK BC terminals and a lower protein density present in a subpopulation of boutons of PV BCs. PMID: 28466358
  2. The two projections from CCK(NTS) neurons reduce food intake through opposite motivational states; one pathway signals positive valence (CCK(NTS)-->paraventricular nucleus of the hypothalamus ) and the other signals negative valence (CCK(NTS)-->parabrachial nucleus). PMID: 28684275
  3. Taken together, these results demonstrate that DMH NPY descending signals affect CCK-induced satiety, at least in part, via modulation of NTS catecholaminergic neuronal signaling. PMID: 27534875
  4. We conclude that only a small fraction of neuronal CCK is nonsulfated. The intracellular distribution of nonsulfated CCK in neurons suggests that they contribute only modestly to the CCK transmitter activity. PMID: 27535680
  5. The favorable treatment strategy for acute pancreatitis is to keep the pancreas at rest during an early stage followed by pancreatic stimulation by promoting endogenous CCK release. PMID: 26167074
  6. Cardiac expression of pro-cholecystokinin is cell-specific, which differentiates the expression from that of intestinal endocrine cells and cerebral neurons. PMID: 25627687
  7. Estrogen-induced anxiolytic effects were associated with changes of the cholecystokinin system in brain regions controlling anxiety-like behavior. PMID: 24732637
  8. All together, this study clearly demonstrates an important protective role of cholecystokinin against sepsis induced by Staphylococcus aureus. PMID: 24487953
  9. Data suggest that Cck (here synthetic peptide fragment Cck 1-8; but not peptide YY 3-36 or glucagon-like peptide 1) mediates anorexic behavioral responses of peripheral sensory neurons (presumably vagal afferent nerves in mucosa of small intestines). PMID: 25117406
  10. CCK-8 octapeptide attenuates the effects of morphine and saline on hippocampal long-term potentiation through CCK2 receptors, suggesting an ameliorative function of CCK-8 on morphine-induced memory impairment. PMID: 24309294
  11. findings suggest that the fibroblast growth factor system is poised to modulate both CCK and FGF-R1 expression in the ventral tegmental area PMID: 24121132
  12. Data suggest that Apo AIV (apolipoprotein AIV) in NTS (nucleus of the solitary tract) or/and peripheral Cck require vagal Cck1r (cholecystokinin receptor 1) signaling to elicit satiation; high-fat diet reduces satiating capacity of these signals. PMID: 24564397
  13. CCK-8 antagonizes electroacpuncture modulation of sympathoexcitatory cardiovascular responses through an opioid mechanism and that inhibition of CCK-8 can convert animals that initially are unresponsive to EA to become responsive. PMID: 23785073
  14. A biliopancreatic diversion model in rats finds a pathophysiological mechanism of action resulting from weight loss and villi elongation. PMID: 22813405
  15. Cholecystokinin has cellular actions within the periaqueductal gray that can both oppose and reinforce opioid and cannabinoid modulation of pain and anxiety within this brain structure. PMID: 21525858
  16. Rats on a medium high-fat diet had significantly higher plasma leptin but lower cholecystokinin levels than rats on a low-fat diet. This corresponded to attenuated or reversed splanchnic nerve responses to cholecystokinin and leptin. PMID: 21239630
  17. Unlike in the dorsal vagal complex (DVC), CCK-8 was only able to activate the myenteric neurons in older rats. This delayed activation compared to the DVC may reflect a delayed role for these neurons in CCK-related functions. PMID: 21093507
  18. High dose lithium reduces the expression of cholecystokinin in hippocampal neurons. PMID: 16535834
  19. Our results provide a cellular and molecular mechanism to explain the roles of CCK in the brain. PMID: 20392936
  20. CaR is the beta 51-63 peptide sensor responsible for the stimulation of CCK secretion in enteroendocrine STC-1 cells. PMID: 19896983
  21. Cholecystokinin induces caspase activation and mitochondrial dysfunction in pancreatic acinar cells PMID: 11964411
  22. Basal endogenous levels of cholecystokinin appear to play an important role in the anxiety-related behaviors in rats. PMID: 12450740
  23. Cholecystokinin has a role in stimulating extracellular signal-regulated kinase through activation of the epidermal growth factor receptor, Yes, and protein kinase C PMID: 12496267
  24. neurons in the rat basolateral amygdala contain cholecystokinin. PMID: 12763251
  25. leptin induces the phosphorylation of extracellular signal-related kinase (ERK)-1/2 proteins and increases cholecystokinin release PMID: 12829630
  26. cholecystokinin system may play important role in expressing the symptopathology of the chronic stress responses such as depression, abnormality of food intake or anxiety-related disorders PMID: 12914981
  27. CCK-IR cells represent one interneuron type that assists in the maturation of glutamatergic synapses (activation of N-methyl-D-aspartate receptors) via GABAergic depolarization of principal cell dendrites. PMID: 12927199
  28. Significant downregulation of CCK transcription is noted in the hippocampus and contralateral cortex 1 day after entorhinal cortex lesioning and an increased signal in ipsilateral cortex, followed by up-regulated CCK mRNA expression at postlesional day 5. PMID: 12946704
  29. Spinal cord injury increases levels of cholecystokinin mRNA in the cortex, diencephalon, and mesencephalon of rats. Animals that developed pain post-injury had higher CCK levels than animals that did not develop pain. PMID: 14559369
  30. The degree of colocalization of the prohormone convertase (PC) enzymes PC1, PC2, and PC5 with CCK in rat brain is regionally specific. PMID: 14608596
  31. in rat retina, CCK induces tyrosine phosphorylation of p130(Cas), in a time and concentration-dependent manner. PMID: 14698681
  32. Plasma levels CCK and CGRP were significantly increased through 3 hours and 7 days postinjury, with the peak at 72 hours. CCK in jejunum changed as in plasma. PMID: 15040036
  33. procholecystokinin undergoes parallel pathways of proteolytic cleavages PMID: 15260493
  34. Data suggest that cholecystokinin may play a role in the generation of negative affective states indexed by 22-kilohertz (kHz) ultrasonic calls in certain regions of the brain. PMID: 15464747
  35. Co-expression patterns of the neuropeptides vasoactive intestinal peptide and cholecystokinin with the transduction molecules alpha-gustducin and T1R2 in rat taste receptor cells. PMID: 15561439
  36. A study of the hypothesis that CCK in the rostral ventromedial medulla may engage descending pain facilitatory pathways to enhance spinal nociceptive transmission and attenuate morphine antinociception. PMID: 15647484
  37. CCK preadministration to obese rats did not affect ghrelin-induced food intake. PMID: 15890776
  38. Elevated CCK levels in the posterior cortex may be related to negative aspects of play, while lower CCK levels in the hypothalamus may reflect the more positive valenced aspects of play during bouts of juvenile social-play fighting. PMID: 16143427
  39. Intra-nucleus accumbens administration of a CCK2 receptor antagonist inhibited not only the development but also the expression of chronic morphine-induced antinociceptive tolerance. PMID: 16837074
  40. stimulation of cyclic adenosine monophosphate-protein kinase A signaling pathway by CCK-8 through CCK-1R and CCK-2R inhibits the LPS-induced activation of p38 kinase and NF-kappaB to block the IL-1beta production in rat pulmonary interstitial macrophages PMID: 17505309
  41. CCK increased the frequency of spontaneous inhibitory postsynaptic potentials and currents. This effect was blocked by tetrodotoxin, indicating that the CCK effect is likely mediated by direct excitation of GABAergic interneurons. PMID: 17904218
  42. These data show that CCK is an initiating factor in acute pancreatitis in the rat. PMID: 18297100
  43. Results show that CCK plays a role in regulating the access of leptin to the brain via CCK1 receptors. PMID: 18587446
  44. Report effects of cholecystokinin-58 on type 1 cholecystokinin receptor function and regulation. PMID: 18776046
  45. Results show that synaptophysin-containing cells co-expressed vesicular-associated membrane protein 2 and cholecystokinin. PMID: 19253017
  46. Duodenal Cholecystokinin requires the activation of the gut Cholecystokinin-A receptor and a gut-brain-liver neuronal axis to lower glucose production. PMID: 19656488
  47. assessed whether leptin signaling in hindbrain also enhances these responses to CCK PMID: 19726710

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Subcellular Location Secreted
Protein Families Gastrin/cholecystokinin family
Tissue Specificity The shortest form (CCK8) is predominantly found in the brain, whereas the larger ones are found in the intestine.
Database Links

KEGG: rno:25298

STRING: 10116.ENSRNOP00000026159

UniGene: Rn.9781

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