Rat Clara cell protein,CC16 ELISA Kit

Instructions
Code CSB-E08681r
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name Clara cell protein,CC16
Alternative Names Scgb1a1 ELISA kit; Cc10 ELISA kit; Ugb ELISA kit; UtgUteroglobin ELISA kit; Clara cell phospholipid-binding protein ELISA kit; CCPBP ELISA kit; Clara cells 10 kDa secretory protein ELISA kit; CC10 ELISA kit; PCB-binding protein ELISA kit; Secretoglobin family 1A member 1 ELISA kit
Abbreviation CC16
Uniprot No. P17559
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 3.12 pg/mL-200 pg/mL
Sensitivity 0.78 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat CC16 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 97
Range % 92-102
1:2 Average % 91
Range % 85-98
1:4 Average % 90
Range % 88-93
1:8 Average % 100
Range % 96-104
Recovery
The recovery of rat CC16 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 90 87-92
EDTA plasma (n=4) 106 103-110
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
200 2.147 2.235 2.191 2.089
100 1.683 1.703 1.693 1.591
50 1.231 1.255 1.243 1.141
25 0.885 0.902 0.894 0.792
12.5 0.502 0.528 0.515 0.413
6.25 0.343 0.359 0.351 0.249
3.12 0.218 0.236 0.227 0.125
0 0.103 0.100 0.102  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function Binds phosphatidylcholine, phosphatidylinositol, polychlorinated biphenyls (PCB) and weakly progesterone, potent inhibitor of phospholipase A2.
Gene References into Functions
  1. The expression level of CC16 in lung tissues and BALF decreased in rat lung tissues exposed to mixed air pollutants. PMID: 18303626
  2. IL-13 induces CCSP expression via an EGFR- and leukocyte-dependent pathway. PMID: 12060562
  3. Results show that secretory markers such as CFTR, CC10, and CC26 are present in taste cells of rat circumvallate papillae, and their immunoreactivity is expressed, to a different extent, in subsets of taste cells that express alpha-gustducin. PMID: 18156603
Subcellular Location Secreted
Protein Families Secretoglobin family
Tissue Specificity Clara cells (nonciliated cells of the surface epithelium of the pulmonary airways).
Database Links

KEGG: rno:25575

STRING: 10116.ENSRNOP00000027342

UniGene: Rn.2206

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