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Rat Clara cell protein,CC16 ELISA Kit

Rat Clara cell protein,CC16 ELISA Kit

^Instructions

Code	CSB-E08681r
Size	96T,5×96T,10×96T
Trial Size
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Target Data

Product Details

Target Name

Clara cell protein,CC16

Alternative Names

Scgb1a1 ELISA kit; Cc10 ELISA kit; Ugb ELISA kit; UtgUteroglobin ELISA kit; Clara cell phospholipid-binding protein ELISA kit; CCPBP ELISA kit; Clara cells 10 kDa secretory protein ELISA kit; CC10 ELISA kit; PCB-binding protein ELISA kit; Secretoglobin family 1A member 1 ELISA kit

Abbreviation

CC16

Uniprot No.

P17559

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, tissue homogenates

Detection Range

3.12 pg/mL-200 pg/mL

Sensitivity

0.78 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Others

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat CC16 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	92-102
1:2	Average %	91
	Range %	85-98
1:4	Average %	90
	Range %	88-93
1:8	Average %	100
	Range %	96-104

Recovery

The recovery of rat CC16 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	87-92
EDTA plasma (n=4)	106	103-110

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
200	2.147	2.235	2.191	2.089
100	1.683	1.703	1.693	1.591
50	1.231	1.255	1.243	1.141
25	0.885	0.902	0.894	0.792
12.5	0.502	0.528	0.515	0.413
6.25	0.343	0.359	0.351	0.249
3.12	0.218	0.236	0.227	0.125
0	0.103	0.100	0.102

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Citations

Clara cells protein, prolactin and transcription factors of protein NF-ĸB and c-Jun/AP-1 levels in rats inhaled to stainless steel welding dust and its soluble form Hałatek T.et al,INTERNATIONAL JOURNAL OF OCCUPATIONAL MEDICINE AND ENVIRONMENTAL HEALTH,2018

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Target Data

Function	Binds phosphatidylcholine, phosphatidylinositol, polychlorinated biphenyls (PCB) and weakly progesterone, potent inhibitor of phospholipase A2.
Gene References into Functions	The expression level of CC16 in lung tissues and BALF decreased in rat lung tissues exposed to mixed air pollutants. PMID: 18303626 IL-13 induces CCSP expression via an EGFR- and leukocyte-dependent pathway. PMID: 12060562 Results show that secretory markers such as CFTR, CC10, and CC26 are present in taste cells of rat circumvallate papillae, and their immunoreactivity is expressed, to a different extent, in subsets of taste cells that express alpha-gustducin. PMID: 18156603
Subcellular Location	Secreted
Protein Families	Secretoglobin family
Tissue Specificity	Clara cells (nonciliated cells of the surface epithelium of the pulmonary airways).
Database Links	KEGG: rno:25575 STRING: 10116.ENSRNOP00000027342 UniGene: Rn.2206

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