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Recombinant Rat Uteroglobin protein (Scgb1a1) (Active)

Recombinant Rat Uteroglobin protein (Scgb1a1) (Active)

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Code	CSB-AP000221RA
Abbreviation	Recombinant Rat Cc10 protein (Active)
MSDS	MSDS Datasheet
Size	$142
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Target Background

Product Details

Purity

>97% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU/μg as determined by LAL method.

Activity

Fully biologically active when compared to standard. The ED50 as determined by the ability of the immobilized protein to support the adhesion of the A549 human lung carcinoma cells is less than 5.0 μg/ml, corresponding to a specific activity of >200 IU/m.

Target Names

Scgb1a1

Uniprot No.

P17559

Research Area

Immunology

Alternative Names

Scgb1a1; Cc10; Ugb; UtgUteroglobin; Clara cell phospholipid-binding protein; CCPBP; Clara cells 10 kDa secretory protein; CC10; PCB-binding protein; Secretoglobin family 1A member 1

Species

Rattus norvegicus (Rat)

Source

E.Coli

Expression Region

20-96aa

Complete Sequence

SSDICPGFLQ VLEALLLGSE SNYEAALKPF NPASDLQNAG TQLKRLVDTL PQETRINIVK LTEKILTSPL CEQDLRV

Mol. Weight

8.5 kDa

Protein Length

Full Length of Mature Protein

Tag Info

Tag-Free

Form

Lyophilized powder

Buffer

Lyophilized from a 0.2 µm filtered 20 mM PB, pH 7.4, 150mM NaCl

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

5-10 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Explore the potential of Recombinant Rat Uteroglobin (Scgb1a1) in your immunology research. This versatile, tag-free secretory protein, expressed in E. coli, covers the 20-96aa region and is the full length of the mature protein. Scgb1a1 boasts a purity of over 97% as determined by SDS-PAGE and HPLC, and endotoxin levels below 1.0 EU/µg, ensuring excellent quality for your experiments. The lyophilized powder demonstrates full biological activity, with an ED₅₀ of less than 5.0 μg/ml as determined by its ability to support the adhesion of A549 human lung carcinoma cells, reflecting a specific activity of more than 200 IU/mg. Enhance your studies with this valuable research tool in immunology.

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Target Background

Function

Binds phosphatidylcholine, phosphatidylinositol, polychlorinated biphenyls (PCB) and weakly progesterone, potent inhibitor of phospholipase A2.

Gene References into Functions

The expression level of CC16 in lung tissues and BALF decreased in rat lung tissues exposed to mixed air pollutants. PMID: 18303626
IL-13 induces CCSP expression via an EGFR- and leukocyte-dependent pathway. PMID: 12060562
Results show that secretory markers such as CFTR, CC10, and CC26 are present in taste cells of rat circumvallate papillae, and their immunoreactivity is expressed, to a different extent, in subsets of taste cells that express alpha-gustducin. PMID: 18156603

Subcellular Location

Secreted.

Protein Families

Secretoglobin family

Tissue Specificity

Clara cells (nonciliated cells of the surface epithelium of the pulmonary airways).

Database Links

KEGG: rno:25575

STRING: 10116.ENSRNOP00000027342

UniGene: Rn.2206

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