Rat Coagulation factor VII(F7) ELISA kit

Instructions
Code CSB-EL007930RA
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name coagulation factor VII (serum prothrombin conversion accelerator)
Alternative Names F7Coagulation factor VII ELISA Kit; EC 3.4.21.21 ELISA Kit; Serum prothrombin conversion accelerator) [Cleaved into: Factor VII light chain; Factor VII heavy chain] ELISA Kit
Abbreviation F7
Uniprot No. Q8K3U6
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.78 ng/mL-50 ng/mL
Sensitivity 0.195 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Blood Coagulation
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat F7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:200 Average % 91    
Range % 86-98    
1:400 Average % 97    
Range % 92-106    
1:800 Average % 93    
Range % 88-99    
1:1600 Average % 89    
Range % 83-95    
Recovery
The recovery of rat F7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 92 87-97    
EDTA plasma (n=4) 109 103-115    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
ng/ml OD1 OD2 Average Corrected    
50 2.468 2.652 2.560 2.424    
25 2.115 2.076 2.096 1.960    
12.5 1.503 1.504 1.504 1.368    
6.25 0.845 0.805 0.825 0.689    
3.12 0.526 0.524 0.525 0.389    
1.56 0.381 0.359 0.370 0.234    
0.78 0.274 0.259 0.267 0.131    
0 0.137 0.135 0.136      
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

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Target Background

Function
(From Uniprot)
Initiates the extrinsic pathway of blood coagulation. Serine protease that circulates in the blood in a zymogen form. Factor VII is converted to factor VIIa by factor Xa, factor XIIa, factor IXa, or thrombin by minor proteolysis. In the presence of tissue factor and calcium ions, factor VIIa then converts factor X to factor Xa by limited proteolysis. Factor VIIa will also convert factor IX to factor IXa in the presence of tissue factor and calcium (By similarity).
Gene References into Functions
  1. The EGF(1) region of rat coagulation factor can mediate the specific binding of FVII with tissue factor. PMID: 18315926
Subcellular Location Secreted
Protein Families Peptidase S1 family
Tissue Specificity Plasma.
Database Links

KEGG: rno:260320

STRING: 10116.ENSRNOP00000038466

UniGene: Rn.86416

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