Rat Cytochrome c(CYCS) ELISA kit

Code CSB-EL006328RA
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Rat CYCS ELISA Kit was designed for the quantitative measurement of Rat CYCS protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 78 pg/mL-5000 pg/mL and the sensitivity is 19.5 pg/mL.

Target Name cytochrome c, somatic
Alternative Names CycsCytochrome c ELISA Kit; somatic ELISA Kit
Abbreviation CYCS
Uniprot No. P62898
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 78 pg/mL-5000 pg/mL
Sensitivity 19.5 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat CYCS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %90
Range %87-94
1:2Average %89
Range %85-96
1:4Average %92
Range %90-95
1:8Average %91
Range %87-96
Recovery
The recovery of rat CYCS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9490-96
EDTA plasma (n=4)9084-96
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
50002.581 2.458 2.520 2.427
25002.234 2.186 2.210 2.117
12501.638 1.621 1.630 1.537
6251.103 1.097 1.100 1.007
3120.692 0.672 0.682 0.589
1560.393 0.386 0.390 0.297
780.236 0.223 0.230 0.137
00.094 0.092 0.093  
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

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Target Background

Function
(From Uniprot)
Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.; Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.
Subcellular Location Mitochondrion intermembrane space. Note=Loosely associated with the inner membrane.
Protein Families Cytochrome c family
Tissue Specificity Found in embryos and in adult liver and heart.
Database Links

KEGG: rno:100363502

STRING: 10116.ENSRNOP00000041521

UniGene: Rn.161841

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