Rat Cytochrome c(CYCS) ELISA kit

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Rat Cytochrome c(CYCS) ELISA kit

Name: Rat Cytochrome c(CYCS) ELISA kit
Brand: CUSABIO
SKU: CSB-EL006328RA

^Instructions

Code	CSB-EL006328RA
Size	96T,5×96T,10×96T
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Target Data

Target Name

cytochrome c, somatic

Alternative Names

CycsCytochrome c ELISA Kit; somatic ELISA Kit

Abbreviation

CYCS

Uniprot No.

P62898

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, tissue homogenates

Detection Range

78 pg/mL-5000 pg/mL

Sensitivity

19.5 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cell Biology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat CYCS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	87-94
1:2	Average %	89
	Range %	85-96
1:4	Average %	92
	Range %	90-95
1:8	Average %	91
	Range %	87-96

Recovery

The recovery of rat CYCS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-96
EDTA plasma (n=4)	90	84-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
5000	2.581	2.458	2.520	2.427
2500	2.234	2.186	2.210	2.117
1250	1.638	1.621	1.630	1.537
625	1.103	1.097	1.100	1.007
312	0.692	0.672	0.682	0.589
156	0.393	0.386	0.390	0.297
78	0.236	0.223	0.230	0.137
0	0.094	0.092	0.093

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

5-7 working days

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Function	Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.; FUNCTION
Subcellular Location	Mitochondrion intermembrane space
Protein Families	Cytochrome c family
Tissue Specificity	Found in embryos and in adult liver and heart.
Database Links	KEGG: rno:100363502 STRING: 10116.ENSRNOP00000041521 UniGene: Rn.161841

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

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