Rat Cytochrome c(CYCS) ELISA kit

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Rat Cytochrome c(CYCS) ELISA kit

Specification
Target

Code

CSB-EL006328RA

See More Details

Product Type

ELISA Kit

Size

96T,5×96T,10×96T

Uniprot No.

P62898

Lead Time

5-7 working days

Abbreviation

CYCS

Protein Biological Process 1

Apoptosis/Autophagy

Target Name

cytochrome c, somatic

Alias

CYC, HCS, THC4, OTTHUMP00000202043|OTTHUMP00000202044|cytochrome c

Species

Rattus norvegicus (Rat)

Protein Biological Process 3

Apoptosis

Sample Types

serum, plasma, tissue homogenates

Detection Range

78 pg/mL-5000 pg/mL

Sensitivity

19.5 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cell Biology

Protocol

Instructions

Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.

Assay Principle

quantitative

Measurement

Sandwich

Target Details

This gene encodes cytochrome c, a component of the electron transport chain in mitochondria. The heme group of cytochrome c accepts electrons from the b-c1 complex and transfers electrons to the cytochrome oxidase complex. Cytochrome c is also involved in initiation of apoptosis. Upon release of cytochrome c to the cytoplasm, the protein binds apoptotic protease activating factor which activates the apoptotic initiator procaspase 9. Many cytochrome c pseudogenes exist, scattered throughout the human genome.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat CYCS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	87-94
1:2	Average %	89
	Range %	85-96
1:4	Average %	92
	Range %	90-95
1:8	Average %	91
	Range %	87-96

Recovery

The recovery of rat CYCS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-96
EDTA plasma (n=4)	90	84-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
5000	2.581	2.458	2.520	2.427
2500	2.234	2.186	2.210	2.117
1250	1.638	1.621	1.630	1.537
625	1.103	1.097	1.100	1.007
312	0.692	0.672	0.682	0.589
156	0.393	0.386	0.390	0.297
78	0.236	0.223	0.230	0.137
0	0.094	0.092	0.093

References

Epigallocatechin-3-gallate protects against diabetic cardiomyopathy through modulating the cardiometabolic risk factors, oxidative stress, inflammation, cell death and fibrosis in streptozotocin-nicotinamide-induced diabetic rats Othman A.et al,Biomed Pharmacother.,/
Ginger alleviates hyperglycemia-induced oxidative stress, inflammation and apoptosis and protects rats against diabetic nephropathy Amir M.Al Hroob.et al,Biomedicine & Pharmacotherapy,July 11
Olea europaea leaf extract up-regulates Nrf2/ARE/HO-1 signaling and attenuates cyclophosphamide-induced oxidative stress, inflammation and apoptosis in rat kidney ALHaithloul HAS, et al,Biomedicine & Pharmacotherapy,January 3
Effect of a chamomile extract in protecting against radiation-induced intestinal mucositis Khayyal MT, et al,Phytotherapy Research,January 10

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Function	Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.; FUNCTION
Subcellular Location	Mitochondrion intermembrane space
Protein Families	Cytochrome c family
Tissue Specificity	Found in embryos and in adult liver and heart.
Database Links	KEGG: rno:100363502 STRING: 10116.ENSRNOP00000041521 UniGene: Rn.161841
Pathway	p53 signaling pathway Apoptosis

Cycs Protein

Cycs Proteins for Rattus norvegicus (Rat)

Code	Product Name	Source
CSB-YP006328RA CSB-EP006328RA CSB-BP006328RA CSB-MP006328RA	Recombinant Rat Cytochrome c, somatic(Cycs)	Yeast E.coli Baculovirus Mammalian cell

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

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