Rat Cytochrome c(CYCS) ELISA kit

Code CSB-EL006328RA
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P62898
Lead Time 5-7 working days
Abbreviation CYCS
Protein Biological Process 1 Apoptosis/Autophagy
Target Name cytochrome c, somatic
Alias CYC, HCS, THC4, OTTHUMP00000202043|OTTHUMP00000202044|cytochrome c
Species Rattus norvegicus (Rat)
Protein Biological Process 3 Apoptosis
Sample Types serum, plasma, tissue homogenates
Detection Range 78 pg/mL-5000 pg/mL
Sensitivity 19.5 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This gene encodes cytochrome c, a component of the electron transport chain in mitochondria. The heme group of cytochrome c accepts electrons from the b-c1 complex and transfers electrons to the cytochrome oxidase complex. Cytochrome c is also involved in initiation of apoptosis. Upon release of cytochrome c to the cytoplasm, the protein binds apoptotic protease activating factor which activates the apoptotic initiator procaspase 9. Many cytochrome c pseudogenes exist, scattered throughout the human genome.
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat CYCS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %90
Range %87-94
1:2Average %89
Range %85-96
1:4Average %92
Range %90-95
1:8Average %91
Range %87-96
Recovery
The recovery of rat CYCS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9490-96
EDTA plasma (n=4)9084-96
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
50002.581 2.458 2.520 2.427
25002.234 2.186 2.210 2.117
12501.638 1.621 1.630 1.537
6251.103 1.097 1.100 1.007
3120.692 0.672 0.682 0.589
1560.393 0.386 0.390 0.297
780.236 0.223 0.230 0.137
00.094 0.092 0.093  
References
Still Have Questions? Leave a Message or Start an on-line Chat
Function Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.; FUNCTION
Subcellular Location Mitochondrion intermembrane space
Protein Families Cytochrome c family
Tissue Specificity Found in embryos and in adult liver and heart.
Database Links

KEGG: rno:100363502

STRING: 10116.ENSRNOP00000041521

UniGene: Rn.161841

Pathway p53 signaling pathway
Apoptosis

Related Products

Cycs Proteins

Cycs Proteins for Rattus norvegicus (Rat)

Most popular with customers

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

Copyright © 2007-2018 www.cusabio.com CUSABIO TECHNOLOGY LLC All Rights Reserved.