Rat Galectin-3(LGALS3) ELISA kit

Code CSB-EL012887RA
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name lectin, galactoside-binding, soluble, 3
Alternative Names Lgals3 ELISA Kit; Galectin-3 ELISA Kit; Gal-3 ELISA Kit; 35 kDa lectin ELISA Kit; Carbohydrate-binding protein 35 ELISA Kit; CBP 35 ELISA Kit; Galactose-specific lectin 3 ELISA Kit; IgE-binding protein ELISA Kit; Laminin-binding protein ELISA Kit; Lectin L-29 ELISA Kit; Mac-2 antigen ELISA Kit
Abbreviation LGALS3
Uniprot No. P08699
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 1.56 ng/mL-100 ng/mL
Sensitivity 0.39 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of rat LGALS3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:1Average %88
Range %84-93
1:2Average %100
Range %95-105
1:4Average %87
Range %83-90
1:8Average %96
Range %93-99
The recovery of rat LGALS3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8782-91
EDTA plasma (n=4)8783-90
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
1002.771 2.798 2.785 2.621
502.066 2.098 2.082 1.918
251.412 1.400 1.406 1.242
12.50.841 0.868 0.855 0.691
6.250.597 0.575 0.586 0.422
3.120.466 0.478 0.472 0.308
1.560.318 0.336 0.327 0.163
00.163 0.165 0.164
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

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Target Background

(From Uniprot)
Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. In the nucleus
Gene References into Functions
  1. Galectin-3 is a crucial factor in inducing pulmonary artery endothelium cell transformation into VSMCs through Jagged1/Notch1 dependent MyoD and SRF expression in this pathological condition PMID: 30463073
  2. that lack of Gal3 worsens subchronic injury after neonatal focal stroke PMID: 27836669
  3. Therapeutic silencing of galectin3 improves cardiomyocyte apoptosis and survival during heart failure. PMID: 29286090
  4. Gal-3 serves as a critical regulator in the pathogenesis of pulmonary arterial hypertension by regulating the proliferation, differentiation, and extracellular matrix deposition synthesis of pulmonary adventitial fibroblast. PMID: 28826890
  5. Gal-3 is overexpressed in aorta and AVs from PO rats. Gal-3 pharmacological inhibition blocks aortic and AV remodeling in experimental PO. Gal-3 could be a new therapeutic approach to delay the progression and the development of aortic remodeling and AV calcification in PO. PMID: 28758988
  6. Gal-3 plays an important role in the pulmonary arterial hypertension-induced right ventricular remodeling through interacting with the Nox4 and Nox4-derived oxidative stress. PMID: 28431936
  7. These findings demonstrate that Gal-3 is required for TGF-beta1-stimulated vascular fibrosis via a STAT3 signaling cascade and that MMP-9 is also involved in TGF-beta1/Gal-3-induced vascular fibrosis. PMID: 27870162
  8. The increase in myocardial Gal-3 expression was associated with cardiac fibrosis and inflammation. PMID: 28360193
  9. galectin-3 expression in the rat brain seems to be regulated by developmental cascades, and functionally and neuroanatomically related brain nuclei constitutively express galectin-3 in adulthood. PMID: 28255782
  10. Data suggest that inhibition of galectin-3 (here, by soluble dietary fiber supplement, modified citrus pectin) prevents initiation/progression of liver cirrhosis by inducing apoptosis in liver stellate cells. PMID: 27010252
  11. Up-regulated galectin-3 signaling might be involved in the pathogenesis in experimental cardiorenal syndrome PMID: 26875907
  12. Findings suggest the involvement of GAL-3 in the glycation and oxidative stress under diabetic conditions and its cytoprotective role in Schwann cells. PMID: 25481849
  13. This study provides a new insight into the molecular mechanisms of HF mediated by PKC-alpha and galectin-3. PMID: 25489662
  14. galectin-3 is activated in microglia and macrophages according to the progression of glioma. PMID: 23179497
  15. Gal-3 is required for inflammatory and fibrotic responses to aldosterone in vascular smooth muscle. PMID: 23117656
  16. Galectin-3 expression is induced in macrophages, particularly ED1+ macrophages, during the course of wound healing. PMID: 21435650
  17. The morphometric analysis showed a significant decrease in the frequency of myelinated axons, myelin turns (lamellae) and g-ratio in the corpus callosum and striatum of Lgals3(-/-) compared with wild-type (WT) mice. PMID: 21566659
  18. Our results identify a cell type-specific control of galectin-3 synthesis by glucocorticoids in lung bronchiolar Clara cells and interstitial macrophages PMID: 21472689
  19. Abnormally lower level of galectin-3 expression and elevated level of rno-let-7d expression were observed in brain of SHR than those in WKY rat. PMID: 20557304
  20. Basal expression and secretion levels of rat gal-3 are higher in glioma cells compared with normal rat primary astrocytes; upregulated interferon-gamma levels in virally infected glioma cells increase gal-3 secretion into the culture media. PMID: 20980634
  21. galectin-3 may be involved in the complex process of kidney regeneration following ischemia/reperfusion injury PMID: 20865664
  22. Inhibition of galectin-3 using RNAi technique can suppress proliferation and induce apoptosis in hepatic stellate cells. PMID: 19785949
  23. MP20 ia a galectin-3 ligand PMID: 11532191
  24. galectin 3 controls proliferation in thyroid cells PMID: 12615069
  25. data suggest complex induction mechanisms of gal-3 expression in neuronally differentiating PC12 cells involving NGF-, but not CNTF- and IL-6-driven (in NGF-primed cells) Ras/MAPK-related signalling pathways PMID: 14622091
  26. Galectin-3 is the major LDN-binding protein in macrophages. Galectin-3 can mediate recognition and phagocytosis of LDN-coated particles by macrophages. PMID: 15265923
  27. An early increase in galectin-3 expression identifies failure-prone hypertrophied hearts. Galectin-3, a macrophage-derived mediator, induces cardiac fibroblast proliferation, collagen deposition, and ventricular dysfunction. PMID: 15520318
  28. Osteoblastic exposure to irreversible advanced glycation endproducts alters their expression and secretion of galectin-3, which could have significant consequences on osteoblast metabolism and thus on bone turnover. PMID: 15646023
  29. Thus galectin-3 may play several important roles in the CCD, including mediating the adaptation of beta-intercalated cells during metabolic acidosis. PMID: 16131647
  30. siRNA knockdown of Galectin-3 inhibited myofibroblast activation after hepatic injury and may therefore provide an alternative therapeutic approach to the prevention and treatment of liver fibrosis PMID: 16549783
  31. Combined proteome-transcriptome-genome and functional analyses identify gal-3 as a candidate gene/protein in Type 1 diabetes. susceptibility PMID: 16600178
  32. galectin-3 promotes K-Ras signaling to both Raf and PI3-K. 3. PMID: 16691442
  33. Galectin-3 is expressed in the neonatal, young, and mature rat intervertebral disc. PMID: 17202886
  34. Galectin-3 was localized to polymorphonuclear neutrophils, microglia, monocytes and macrophages, suggesting an involvement of galectin-3 in the neuroinflammatory processes leading to brain damage in PM. PMID: 17706429
  35. Abundant galectin-3 observed in the area of severe bone destruction may act as a negative regulator for the upregulated osteoclastogenesis accompanying inflammation to prevent excess bone destruction. PMID: 19015643
  36. These results suggest that Gal-3 expressed by activated microglia/infiltrating macrophages and astrocytes in the ischemic brain may play a role in post-ischemic tissue remodeling by enhancing angiogenesis and neurogenesis. PMID: 19573520

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Subcellular Location Cytoplasm, Nucleus, Secreted
Database Links

KEGG: rno:83781

STRING: 10116.ENSRNOP00000014216

UniGene: Rn.764


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