Rat Gamma-glutamyltranspeptidase 1(GGT1) ELISA kit

Code CSB-EL009394RA
Size 96T,5×96T,10×96T
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Product Details

Target Name
gamma-glutamyltransferase 1
Alternative Names
Ggt1; Ggt; Glutathione hydrolase 1 proenzyme; Gamma-glutamyltransferase 1; Gamma-glutamyltranspeptidase 1; GGT 1; Leukotriene-C4 hydrolase; CD antigen CD224
Abbreviation
GGT1
Uniprot No.
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates
Detection Range
4.7 mIU/mL-300 mIU/mL
Sensitivity
1.2 mIU/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Metabolism
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat GGT1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %97
Range %92-103
1:2Average %90
Range %85-94
1:4Average %92
Range %88-99
1:8Average %89
Range %84-93
Recovery
The recovery of rat GGT1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9288-96
EDTA plasma (n=4)9590-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
mIU/ml OD1OD2AverageCorrected
3002.379 2.311 2.345 2.252
1501.681 1.726 1.704 1.611
751.205 1.224 1.215 1.122
37.50.704 0.726 0.715 0.622
18.80.342 0.317 0.330 0.237
9.40.210 0.199 0.205 0.112
4.70.125 0.118 0.122 0.029
00.090 0.095 0.093
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Rat GGT1 ELISA Kit was designed for the quantitative measurement of Rat GGT1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 4.7 mIU/mL-300 mIU/mL and the sensitivity is 1.2 mIU/mL.

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Target Background

Function
(From Uniprot)
Cleaves the gamma-glutamyl bond of extracellular glutathione (gamma-Glu-Cys-Gly), glutathione conjugates and other gamma-glutamyl compounds, such as leukotriene C4 (LTC4). The metabolism of glutathione by GGT1 releases free glutamate and the dipeptide cysteinyl-glycine, which is hydrolyzed to cysteine and glycine by dipeptidases. In the presence of high concentrations of dipeptides and some amino acids, can also catalyze a transpeptidation reaction, transferring the gamma-glutamyl moiety to an acceptor amino acid to form a new gamma-glutamyl compound. Contributes to cysteine homeostasis, glutathione homeostasis and in the conversion of the leukotriene LTC4 to LTD4.
Gene References into Functions
  1. an extensive ductular reaction in advanced Hepatocellular carcinoma (HCC) and a strong correlation between PCK7 and levels of gammaGT and the poor prognosis and aggressiveness of HCC, is reported. PMID: 27525410
  2. These data demonstrate the important role of endothelial GGT activity in mediating the vasorelaxant effect of S-nitrosoglutathione in rat aorta . PMID: 22984412
  3. The expression of gamma-glutamyltransferase in rat colon carcinoma cells is distinctly regulated during differentiation and oxidative stress PMID: 12030384
  4. Radiation of CC531 colon carcinoma cells blocked cell proliferation, increased cellular size, initiated apoptosis and upregulated GGT activity and protein levels in a dose- and time-related manner. PMID: 16386375
  5. With the co-existence of inflammation and hypoxia in the brain after ischemic stroke, dynamic changes of gammaGT activity reflect evolving changes of endothelial function. PMID: 16973162
  6. Data suggest that extracellular glutathione elicits gamma-glutamyl transpeptidase and ROS-dependent transactivation of tyrosine kinase signaling that upregulates potassium channel activity or expression via redox-mediated mechanisms. PMID: 19419996
  7. The acylation reaction of GGT by gamma-glutamyl anilides can apparently be accelerated by either electron-withdrawing or electron-donating substituents, suggesting a concerted mechanism involving kinetic general acid catalysis of aniline departure. PMID: 11601992

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Subcellular Location
Cell membrane; Single-pass type II membrane protein.
Protein Families
Gamma-glutamyltransferase family
Tissue Specificity
Detected in adult kidney and mammary gland, and in fetal liver.
Database Links

KEGG: rno:116568

UniGene: Rn.10010

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