Recombinant Rat Gamma-glutamyltranspeptidase 1 (Ggt1)

Code CSB-CF009394RA
MSDS
Size Pls inquire
Source in vitro E.coli expression system
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Product Details

Target Names
Ggt1
Uniprot No.
Alternative Names
Ggt1; Ggt; Glutathione hydrolase 1 proenzyme; Gamma-glutamyltransferase 1; Gamma-glutamyltranspeptidase 1; GGT 1; Leukotriene-C4 hydrolase; CD antigen CD224
Species
Rattus norvegicus (Rat)
Expression Region
1-379
Target Protein Sequence
MKNRFLVLGLVAVVLVFVIIGLCIWLPTTSGKPDHVYSRAAVATDAKRCSEIGRDMLQEGGSVVDAAIASLLCMGLINAHSMGIGGGLFFTIYNSTTRKAEVINAREMAPRLANTSMFNNSKDSEEGGLSVAVPGEIRGYELAHQRHGRLPWARLFQPSIQLARHGFPVGKGLARALDKKRDIIEKTPALCEVFCRQGKVLQEGETVTMPKLADTLQILAQEGARAFYNGSLTAQIVKDIQEAGGIMTVEDLNNYRAEVIEHPMSIGLGDSTLYVPSAPLSGPVLILILNILKGYNFSPKSVATPEQKALTYHRIVEAFRFAYAKRTMLGDPKFVDVSQVIRNMSSEFYATQLRARITDETTHPTAYYEPEFYLPDDGG
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Cleaves the gamma-glutamyl bond of extracellular glutathione (gamma-Glu-Cys-Gly), glutathione conjugates and other gamma-glutamyl compounds, such as leukotriene C4 (LTC4). The metabolism of glutathione by GGT1 releases free glutamate and the dipeptide cysteinyl-glycine, which is hydrolyzed to cysteine and glycine by dipeptidases. In the presence of high concentrations of dipeptides and some amino acids, can also catalyze a transpeptidation reaction, transferring the gamma-glutamyl moiety to an acceptor amino acid to form a new gamma-glutamyl compound. Contributes to cysteine homeostasis, glutathione homeostasis and in the conversion of the leukotriene LTC4 to LTD4.
Gene References into Functions
  1. an extensive ductular reaction in advanced Hepatocellular carcinoma (HCC) and a strong correlation between PCK7 and levels of gammaGT and the poor prognosis and aggressiveness of HCC, is reported. PMID: 27525410
  2. These data demonstrate the important role of endothelial GGT activity in mediating the vasorelaxant effect of S-nitrosoglutathione in rat aorta . PMID: 22984412
  3. The expression of gamma-glutamyltransferase in rat colon carcinoma cells is distinctly regulated during differentiation and oxidative stress PMID: 12030384
  4. Radiation of CC531 colon carcinoma cells blocked cell proliferation, increased cellular size, initiated apoptosis and upregulated GGT activity and protein levels in a dose- and time-related manner. PMID: 16386375
  5. With the co-existence of inflammation and hypoxia in the brain after ischemic stroke, dynamic changes of gammaGT activity reflect evolving changes of endothelial function. PMID: 16973162
  6. Data suggest that extracellular glutathione elicits gamma-glutamyl transpeptidase and ROS-dependent transactivation of tyrosine kinase signaling that upregulates potassium channel activity or expression via redox-mediated mechanisms. PMID: 19419996
  7. The acylation reaction of GGT by gamma-glutamyl anilides can apparently be accelerated by either electron-withdrawing or electron-donating substituents, suggesting a concerted mechanism involving kinetic general acid catalysis of aniline departure. PMID: 11601992

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Subcellular Location
Cell membrane; Single-pass type II membrane protein.
Protein Families
Gamma-glutamyltransferase family
Tissue Specificity
Detected in adult kidney and mammary gland, and in fetal liver.
Database Links

KEGG: rno:116568

UniGene: Rn.10010

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