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| Code | CSB-E13036r |
| Size | 96T,5×96T,10×96T |
| Price | Request a Quote or Start an on-line Chat |
| Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
| To assess the linearity of the assay, samples were spiked with high concentrations of rat Hb in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:5 | Average % | 101 | |||||
| Range % | 95-107 | ||||||
| 1:10 | Average % | 93 | |||||
| Range % | 89-97 | ||||||
| 1:20 | Average % | 95 | |||||
| Range % | 90-100 | ||||||
| 1:40 | Average % | 90 | |||||
| Range % | 85-97 | ||||||
| The recovery of rat Hb spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 89 | 84-93 | |||||
| EDTA plasma (n=4) | 92 | 88-96 | |||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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The rat hemoglobin (Hb) ELISA Kit is engineered for accurate measurement of rat hemoglobin levels from samples including serum, plasma, or lysate for RBC. It uses the Competitive-ELISA mechanism in combination with the enzyme-substrate chromogenic reaction to measure the rat hemoglobin content in the sample. The color intensity is negatively correlated with hemoglobin content in the sample. This kit has been validated against standards of sensitivity, specificity, precision, linearity, recovery, and lot-to-lot consistency.
Hemoglobin is a two-way respiratory carrier, transporting oxygen from the lungs to the tissues and facilitating the return transport of carbon dioxide. In arterial circulation, hemoglobin has a high affinity for oxygen and a low affinity for carbon dioxide, organic phosphates, and hydrogen and chloride ions. In addition to being a molecular heat transducer through its oxygenation-deoxygenation cycle as well as a modulator of erythrocyte metabolism, hemoglobin's oxidation is an onset of erythrocyte senescence.It also mediates reactive oxygen and nitrogen species detoxification.p>
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