Rat Immunoglobulin M,IgM ELISA Kit

Code CSB-E07978r
Size 96T,5×96T,10×96T How to order?
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Rat IgM ELISA Kit was designed for the quantitative measurement of Rat IgM protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 6.25 ng/mL-1600 ng/mL and the sensitivity is 6.25 ng/mL .

Target Name Immunoglobulin M,IgM
Abbreviation IgM
Species Rattus norvegicus (Rat)
Sample Types serum, plasma
Detection Range 6.25 ng/mL-1600 ng/mL
Sensitivity 6.25 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat IgM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1000Average %99
Range %95-104
1:2000Average %90
Range %86-95
1:4000Average %89
Range %83-93
1:8000Average %100
Range %95-105
Recovery
The recovery of rat IgM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9492-97
EDTA plasma (n=4)9389-97
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1OD2Average
16000.234 0.227 0.231
4000.433 0.412 0.423
1000.678 0.669 0.674
251.256 1.271 1.264
6.251.698 1.784 1.741
02.031 2.049 2.040
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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