Rat Immunoglobulin M,IgM ELISA Kit

Code CSB-E07978r
Size 96T,5×96T,10×96T
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now
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Product Details

Target Name
Immunoglobulin M,IgM
Rattus norvegicus (Rat)
Sample Types
serum, plasma
Detection Range
6.25 ng/mL-1600 ng/mL
6.25 ng/mL
Assay Time
Sample Volume
Detection Wavelength
450 nm
Research Area
Assay Principle
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of rat IgM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:1000Average %99
Range %95-104
1:2000Average %90
Range %86-95
1:4000Average %89
Range %83-93
1:8000Average %100
Range %95-105
The recovery of rat IgM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9492-97
EDTA plasma (n=4)9389-97
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1OD2Average
16000.234 0.227 0.231
4000.433 0.412 0.423
1000.678 0.669 0.674
251.256 1.271 1.264
6.251.698 1.784 1.741
02.031 2.049 2.040
and FAQs
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days

This Rat IgM ELISA Kit was designed for the quantitative measurement of Rat IgM protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 6.25 ng/mL-1600 ng/mL and the sensitivity is 6.25 ng/mL .

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