Rat Insulin-like Growth Factors 1 Receptor,IGF-1R ELISA Kit

Code CSB-E13873r
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P24062
Lead Time 3-5 working days
Abbreviation IGF1R
Target Name insulin-like growth factor 1 receptor
Alias CD221, IGFIR, IGFR, JTK13, MGC142170, MGC142172, MGC18216, soluble IGF1R variant 1|soluble IGF1R variant 2
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 3.12 pg/mL-200 pg/mL
Sensitivity 0.8 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival.
HGNC 5465
RGD 2869
MGI 96433
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat IGF-1R in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:100 Average % 85  
Range % 81-89  
1:200 Average % 92  
Range % 87-98  
1:400 Average % 100  
Range % 97-103  
1:800 Average % 96  
Range % 91-99  
Recovery
The recovery of rat IGF-1R spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 110 107-113  
EDTA plasma (n=4) 89 85-93  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
200 2.406 2.461 2.434 2.278  
100 1.913 1.946 1.930 1.774  
50 1.526 1.534 1.530 1.374  
25 1.009 1.068 1.039 0.883  
12.5 0.684 0.698 0.691 0.535  
6.25 0.366 0.368 0.367 0.211  
3.12 0.257 0.271 0.264 0.108  
0 0.154 0.157 0.156    
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Function Receptor tyrosine kinase which mediates actions of insulin-like growth factor 1 (IGF1). Binds IGF1 with high affinity and IGF2 and insulin (INS) with a lower affinity. The activated IGF1R is involved in cell growth and survival control. IGF1R is crucial for tumor transformation and survival of malignant cell. Ligand binding activates the receptor kinase, leading to receptor autophosphorylation, and tyrosines phosphorylation of multiple substrates, that function as signaling adapter proteins including, the insulin-receptor substrates (IRS1/2), Shc and 14-3-3 proteins. Phosphorylation of IRSs proteins lead to the activation of two main signaling pathways
Subcellular Location Cell membrane, Single-pass type I membrane protein
Protein Families Protein kinase superfamily, Tyr protein kinase family, Insulin receptor subfamily
Database Links

KEGG: rno:25718

STRING: 10116.ENSRNOP00000019267

UniGene: Rn.10957

Pathway HIF-1 signaling pathway
MAPK signaling pathway
mTOR signaling pathway
PI3K-Akt signaling pathway
Focal adhesion
Adherens junction
Autophagy
Ras signaling pathway
Signaling pathways regulating pluripotency of stem cells
AMPK signaling
FoxO signaling pathway
Rap1 signaling pathway
Endocytosis

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