IGF1R

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Activity
Measured by its binding ability in a functional ELISA. Immobilized Human IGF1R at 2 μg/ml can bind Anti-IGF1R recombinant antibody (CSB-RA011087MA2HU). The EC₅₀ is 1.479-1.702 ng/mL.

The purity of IGF1R was greater than 90% as determined by SEC-HPLC

The Binding Activity of Human IGF1R with Anti-IGF1R Recombinant Antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Human IGF1R (CSB-MP011087HU1) at 2 μg/mL can bind Anti-IGF1R recombinant antibody. The EC₅₀ is 1.479-1.702 ng/mL.

IHC image of CSB-RA011087MA2HU diluted at 1:50 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Anti-Human lgG, Fcy Fragment Specific labeled by HRP and visualized using 0.05% DAB.

IHC image of CSB-RA011087MA2HU diluted at 1:50 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Anti-Human lgG, Fcy Fragment Specific labeled by HRP and visualized using 0.05% DAB.

Immunofluorescence staining of MCF-7 cell with CSB-RA011087MA2HU at 1:30 ,counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Fluorescein (FITC) AffiniPure Goat Anti-Human IgG, Fcγ fragment specific.

Immunofluorescence staining of SH-SY5Y cell with CSB-RA011087MA2HU at 1:30 ,counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Fluorescein (FITC) AffiniPure Goat Anti-Human IgG, Fcγ fragment specific.

Overlay Peak curve showing Hela cells stained with CSB-RA011087MA2HU (red line) at 1:100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was Fluorescein (FITC) AffiniPure Goat Anti-Human IgG, Fcγ fragment specific at 1:200 dilution for 35 min at 4℃.Control antibody (green line) was mouse IgG2a (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.