Rat Insulin-like growth factor 2,IGF-2 ELISA Kit

Code CSB-E04585r
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P01346
Lead Time 3-5 working days
Protein Biological Process 2 glyconeogenesis and glycometabolism
Abbreviation IGF2
Protein Biological Process 1 Biosynthesis/Metabolism
Target Name insulin-like growth factor 2 (somatomedin A)
Alias C11orf43, FLJ22066, FLJ44734, INSIGF, pp9974, OTTHUMP00000011018|OTTHUMP00000011157|insulin-like growth factor 2|insulin-like growth factor II|insulin-like growth factor type 2|putative insulin-like
Species Rattus norvegicus (Rat)
Protein Biological Process 3 Carbohydrate metabolism
Sample Types serum, plasma, tissue homogenates
Detection Range 78 pg/mL-5000 pg/mL
Sensitivity 19.5 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This gene encodes a member of the insulin family of polypeptide growth factors that is involved in development and growth. It is an imprinted gene and is expressed only from the paternally inherited allele. It is a candidate gene for eating disorders. There is a read-through, INS-IGF2, which aligns to this gene at the 3 region and to the upstream INS gene at the 5 region. Alternatively spliced transcript variants, encoding either the same or different isoform, have been found for this gene.
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat IGF-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:100Average %86
Range %83-89
1:200Average %99
Range %85-104
1:400Average %92
Range %88-96
1:800Average %87
Range %85-89
Recovery
The recovery of rat IGF-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 105101-109
EDTA plasma (n=4)9794-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
50002.402 2.459 2.431 2.329
25002.139 2.145 2.142 2.040
12501.674 1.662 1.668 1.566
6251.085 1.098 1.092 0.990
3120.538 0.548 0.543 0.441
1560.314 0.326 0.320 0.218
780.196 0.186 0.191 0.089
00.101 0.103 0.102
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Function The insulin-like growth factors possess growth-promoting activity. Major fetal growth hormone in mammals. Plays a key role in regulating fetoplacental development. IGF-II is influenced by placental lactogen. Also involved in tissue differentiation. Positively regulates myogenic transcription factor MYOD1 function by facilitating the recruitment of transcriptional coactivators, thereby controlling muscle terminal differentiation. In adults, involved in glucose metabolism in adipose tissue, skeletal muscle and liver.
Subcellular Location Secreted
Protein Families Insulin family
Database Links

KEGG: rno:24483

STRING: 10116.ENSRNOP00000047037

UniGene: Rn.118681

Pathway MAPK signaling pathway
PI3K-Akt signaling pathway
Ras signaling pathway

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