Rat Insulin-like growth factor binding protein 2,IGFBP-2 ELISA kit

Code CSB-E07351r
Size 96T,5×96T,10×96T
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Product Details

Target Name
insulin-like growth factor binding protein 2, 36kDa
Alternative Names
Igfbp2 ELISA Kit; Igfbp-2Insulin-like growth factor-binding protein 2 ELISA Kit; IBP-2 ELISA Kit; IGF-binding protein 2 ELISA Kit; IGFBP-2 ELISA Kit; BRL-BP ELISA Kit
Abbreviation
IGFBP2
Uniprot No.
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.625 ng/mL-40 ng/mL
Sensitivity
0.156 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat IGFBP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:20 Average % 107
Range % 95-111
1:40 Average % 104
Range % 95-108
1:80 Average % 90
Range % 83-94
1:160 Average % 97
Range % 87-103
Recovery
The recovery of rat IGFBP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 90 82-94
EDTA plasma (n=4) 101 90-105
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
40 2.631 2.568 2.600 2.439
20 2.014 1.964 1.989 1.828
10 1.126 1.083 1.105 0.944
5 0.589 0.567 0.578 0.417
2.5 0.347 0.356 0.352 0.191
1.25 0.266 0.277 0.272 0.111
0.625 0.219 0.212 0.216 0.055
0 0.159 0.163 0.161  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

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Target Background

Function
(From Uniprot)
Inhibits IGF-mediated growth and developmental rates. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
Gene References into Functions
  1. Insulin-like growth factor 2 (IGF2) is an important regulator of fetal growth. PMID: 24352370
  2. Abnormal expression of IGFBP-2 correlates with hyperoxia-induced lung injury in neonatal rats. PMID: 18549709
  3. Depolarization induces insulin-like growth factor binding protein-2 expression in vivo via NMDA receptor stimulation. PMID: 11914028
  4. results show that liver growth and cell proliferation in association with insulin-like growth factor-I and insulin-like growth factor binding proteins 1 and 2 levels are affected in utero by fetal undernutrition PMID: 12902319
  5. In proliferating astrocytes, IGFBP-2 may help target insulin-like growth factors (IGFs) to IGF-1 receptors, and IGFBP-2 proteases may play a role in enhancing the availability of IGFs. PMID: 15248291
  6. cathepsin L created a 14-kDa carboxyl IGFBP-2 fragment that corresponded to the sole antigen detected in IEC-18 cell lysates as well as a 16.5-kDa fragment found in the media PMID: 15705658
  7. retinoic acid modulates the secreted IGFBP-2 and -3 during O(2) exposure and inhibits the increase in collagen that occurs during lung injury PMID: 16055936
  8. Growth inhibition, which was supported by the strong induction of p21CIP1/WAF1, was correlated with increased secretion of IGFBP-2 and, unexpectedly, with its increased localization in the nucleus and particularly in the cytoplasm. PMID: 16131350
  9. IGFBP-2 plays a role in the lamellar bone formation process. PMID: 17308996
  10. In conclusion, MMP-9-induced IGFBP2 proteolysis may be regarded as an important post-translational event involved in astrocytoma aggressiveness. These new findings support drug targeting of MMP-9 as an interesting approach in the treatment of PMID: 18563800
  11. A brain region- and cell type-specific responses to estrogen in rat brain, suggesting that Igf signaling may mediate the estrogen function in the hippocampus. PMID: 18946176

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Subcellular Location
Secreted.
Tissue Specificity
In adults, expressed in brain, testes, ovaries, and kidney. Expression in the adult liver is barely detectable.
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