The product CSB-E07454r is a ready-to-use microwell, strip plate ELISA Kit for quantitative analysis of rat interleukin 13(IL-13)in biological samples, including serum, plasma, cell culture supernates, and tissue homogenates. Based on the Sandwich-ELISA technique in combination with the enzyme-substrate chromogenic reaction, the color intensity is proportional tothe amount of IL-13 bound in the initial step. IL-13 levels can be calculated according to the standard curve.
This rat IL-13 ELISA Kit has been verified by multiple tests with high sensitivity, strong specificity, precision less than 10%, high recovery, and lot-to-lot consistency.
IL-13 is a pleiotropic cytokine produced by activated Th2 cells, lymphoid cells (ILC2s), mast cells, eosinophils, basophils, macrophages, and B cells. It acts through the IL-13Ra1/IL-4Ra complex to induce activation responses which contribute to the inflammatory diseases. IL-13 and the closely related cytokine IL-4 shares many immunoregulatory functions, including regulation of antibody production and inflammation. It acts as a central mediator in allergy and antihelminth defense with various effects. Besides, IL-13 also mediates goblet cell hyperplasia, airway smooth muscle contractility, collagen deposition, and fibrosis.