Rat Interleukin 13,IL-13 ELISA KIT

Instructions
Code CSB-E07454r
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

The product CSB-E07454r is a ready-to-use microwell, strip plate ELISA Kit for quantitative analysis of rat interleukin 13(IL-13)in biological samples, including serum, plasma, cell culture supernates, and tissue homogenates. Based on the Sandwich-ELISA technique in combination with the enzyme-substrate chromogenic reaction, the color intensity is proportional tothe amount of IL-13 bound in the initial step. IL-13 levels can be calculated according to the standard curve.

This rat IL-13 ELISA Kit has been verified by multiple tests with high sensitivity, strong specificity, precision less than 10%, high recovery, and lot-to-lot consistency.

IL-13 is a pleiotropic cytokine produced by activated Th2 cells, lymphoid cells (ILC2s), mast cells, eosinophils, basophils, macrophages, and B cells. It acts through the IL-13Ra1/IL-4Ra complex to induce activation responses which contribute to the inflammatory diseases. IL-13 and the closely related cytokine IL-4 shares many immunoregulatory functions, including regulation of antibody production and inflammation. It acts as a central mediator in allergy and antihelminth defense with various effects. Besides, IL-13 also mediates goblet cell hyperplasia, airway smooth muscle contractility, collagen deposition, and fibrosis.

Target Name interleukin 13
Alternative Names Il13 ELISA Kit; Il-13 ELISA Kit; Interleukin-13 ELISA Kit; IL-13 ELISA Kit; T-cell activation protein P600 ELISA Kit
Abbreviation IL13
Uniprot No. P42203
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 7.8 pg/mL-500 pg/mL
Sensitivity 1.95 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-13 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 91  
Range % 88-93  
1:2 Average % 104  
Range % 99-107  
1:4 Average % 93  
Range % 88-97  
1:8 Average % 99  
Range % 91-103  
Recovery
The recovery of rat IL-13 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 89-98  
EDTA plasma (n=4) 94 91-96  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
500 1.854 1.766 1.810 1.727  
250 1.117 1.106 1.112 1.029  
125 0.728 0.743 0.736 0.653  
62.5 0.517 0.534 0.526 0.443  
31.2 0.303 0.312 0.308 0.225  
15.6 0.210 0.202 0.206 0.123  
7.8 0.171 0.164 0.168 0.085  
0 0.084 0.082 0.083    
Materials provided
  • A micro ELISA plate --The 96-well plate has been pre-coated with an anti-rat IL-13 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
  • Two vials lyophilized standard --Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
  • One vial Biotin-labeled IL-13 antibody (100 x concentrate) (120 μl/bottle) --Act as the detection antibody.
  • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
  • One vial Biotin-antibody Diluent (15 ml/bottle) --Dilute the high concentration Biotin-antibody to an appropriate working solution.
  • One vial HRP-avidin Diluent (15 ml/bottle) --Dilute the high concentration HRP-avidin solution to an appropriate solution.
  • One vial Sample Diluent (50 ml/bottle)--Dilute the sample to an appropriate concentration.
  • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) --- Wash away unbound or free substances.
  • One vial TMB Substrate (10 ml/bottle) --Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
  • One vial Stop Solution (10 ml/bottle) --Stop the color reaction. The solution color immediately turns from blue to yellow.
  • Four Adhesive Strips (For 96 wells) --Cover the microplate when incubation.
  • An instruction manual
Materials not provided
  • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
  • An incubator can provide stable incubation conditions up to 37°C±5°C.
  • Centrifuge
  • Vortex
  • Squirt bottle, manifold dispenser, or automated microplate washer
  • Absorbent paper for blotting the microtiter plate
  • 50-300ul multi-channel micropipette
  • Pipette tips
  • Single-channel micropipette with different ranges
  • 100ml and 500ml graduated cylinders
  • Deionized or distilled water
  • Timer
  • Test tubes for dilution
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function Cytokine. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses. Positively regulates IL31RA expression in macrophages.
Gene References into Functions
  1. SUMF2 may mediate airway inflammation in allergic asthma by modulating the expression of IL-13 PMID: 26722390
  2. IL-13 significantly enhanced C/EBP-alpha/COX-2 expression and PGE2 production in LPS-treated microglial cells. Paradoxically, IL-13 abolished C/EBP-beta/PPAR-gamma/HO-1 expression. PMID: 23881867
  3. asthma the ERK expression and activation levels were increased, as was the protein level of IL-13. The ERK signaling pathway may be involved in the increased expression of the Th2 cytokine IL-13 in asthma. PMID: 20819635
  4. The IL-13 system is a novel regulator of paSMC growth. Dysregulation of IL-13 receptor expression in IPAH may partially underlie smooth muscle hypertrophy associated with pathological vascular remodeling in IPAH. PMID: 20522789
  5. IL-13 is involved in tobacco smoke-induced changes in the structure and function of rat intrapulmonary airways PMID: 19783789
  6. This finding provides an unequivocal evidence of IL-13 participation in pancreatic fibrosis, illustrating a new strategy for chronic pancreatitis. PMID: 20100461
  7. Inhibition of HO-1 with tin protoporphyrin reversed cytoprotective/antiapoptotic effects of IL-13 in liver transplantation. PMID: 12919086
  8. IL-13 may control brain inflammation by inducing the death of activated microglia in vivo, resulting in an enhancement of neuronal survival. PMID: 15042582
  9. study demonstrated at the protein and mRNA levels, both in vivo and in vitro, that alveolar macrophages produce IL-25 and IL-13 in response to fine particles of TiO2 PMID: 15961726
  10. IL-13 overexpression in the rat could lead to podocyte injury and minimal-change-like nephropathy. PMID: 17429054
  11. The findings support a role for IL-13 as an in vivo antiangiogenic factor and provide a rationale for its use in RA to control. pathologic neovascularization. PMID: 17665443
  12. IL-13 down-regulates the cytokine-induced iNOS transcription by decreasing iNOS specific IRF-1/ISRE binding activity PMID: 17723228
  13. expression decreased in lung in response to alcohol exposure PMID: 19120067
  14. Endogenous IL-13 triggers microglial NADPH oxidase-derived oxidative stress, leading to degeneration of hippocampal neurons in vivo. PMID: 19752235

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Subcellular Location Secreted
Protein Families IL-4/IL-13 family
Database Links

KEGG: rno:116553

STRING: 10116.ENSRNOP00000010121

UniGene: Rn.9921

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