Rat Interleukin 17,IL-17 ELISA KIT

Instructions
Code CSB-E07451r
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name interleukin 17A
Alternative Names Il17a ELISA Kit; Ctla8 ELISA Kit; Il17Interleukin-17A ELISA Kit; IL-17 ELISA Kit; IL-17A ELISA Kit; Cytotoxic T-lymphocyte-associated antigen 8 ELISA Kit; CTLA-8 ELISA Kit
Abbreviation IL17A
Uniprot No. Q61453
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 93.75 pg/mL-6000 pg/mL
Sensitivity 23.43 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-17 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 87  
Range % 80-95  
1:2 Average % 85  
Range % 80-95  
1:4 Average % 90  
Range % 86-100  
1:8 Average % 92  
Range % 86-97  
Recovery
The recovery of rat IL-17 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 90 85-98  
EDTA plasma (n=4) 93 88-101  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
6000 1.865 1.802 1.834 1.739  
3000 1.418 1.376 1.397 1.302  
1500 1.036 1.004 1.020 0.925  
750 0.624 0.589 0.607 0.512  
375 0.379 0.364 0.372 0.277  
187.5 0.211 0.209 0.210 0.115  
93.75 0.132 0.122 0.127 0.032  
0 0.099 0.091 0.095    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

Target Data

Function Ligand for IL17RA and IL17RC. The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC. Involved in inducing stromal cells to produce proinflammatory and hematopoietic cytokines.
Subcellular Location Secreted
Protein Families IL-17 family
Database Links

STRING: 10116.ENSRNOP00000016664

UniGene: Rn.218513

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