Rat Neurofilament light polypeptide(NEFL) ELISA kit

Code CSB-EL015688RA
Size 96T,5×96T,10×96T
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Product Details

Target Name
neurofilament, light polypeptide
Alternative Names
Nefl ELISA Kit; Nf68 ELISA Kit; Nfl ELISA Kit; Neurofilament light polypeptide ELISA Kit; NF-L ELISA Kit; 68 kDa neurofilament protein ELISA Kit; Neurofilament triplet L protein ELISA Kit
Abbreviation
NEFL
Uniprot No.
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates
Detection Range
7.8 pg/mL-500 pg/mL
Sensitivity
1.95 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Neuroscience
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat NEFL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %94
Range %85-98
1:2Average %107
Range %97-111
1:4Average %90
Range %83-94
1:8Average %97
Range %91-102
Recovery
The recovery of rat NEFL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9082-94
EDTA plasma (n=4)9991-104
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
5002.516 2.411 2.464 2.287
2502.052 1.987 2.020 1.843
1251.297 1.282 1.290 1.113
62.50.725 0.784 0.755 0.578
31.20.442 0.437 0.440 0.263
15.60.338 0.330 0.334 0.157
7.80.259 0.249 0.254 0.077
00.178 0.176 0.177
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Rat NEFL ELISA Kit was designed for the quantitative measurement of Rat NEFL protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 7.8 pg/mL-500 pg/mL and the sensitivity is 1.95 pg/mL.

Customer Reviews and Q&A

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 Q&A
Q:

I have a customer inquiring about CSB-EL015688RA. Could you let me know whether any preservatives are contained in each component? Could you also let me know whether it was contained recombinant protein in
this kit? If yes, Please let me know expression system of the recombinant protein.

A:
Thanks for your inquiry!
CSB-EL015688RA
Standard is NS0-derived recombinant protein.
Preservative is liquid proclin 300(1:3000).

Target Background

Function
(From Uniprot)
Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks.
Gene References into Functions
  1. Furthermore, the effect of CaMKP on self-assembly of NFL was examined. Electron microscopy revealed that CaMKP markedly prevented NFL from forming large filamentous aggregates, suggesting that CaMKP-binding to NFL inhibits its filament association. These findings may provide new insights into a novel mechanism for regulating network formation of neurofilaments during neuronal differentiation. PMID: 27369073
  2. Data suggest that tetrahydropapaveroline (an endogenous catechol) causes oxidative stress resulting in astrocyte/neuronal cell death via generation of reactive oxygen species and modification/aggregation of NF-L (as in neurodegenerative diseases). PMID: 23228886
  3. In cultured cortical neurons, hamartin and tuberin co-localize with neurofilament light chain preferentially in the proximal to central growth cone region PMID: 12226091
  4. The amount of NF-L correlated with the morphological retinal and optic nerve damage in rats PMID: 15193534
  5. Increased neuritic degeneration was observed in neuronal cells overexpressing neurofilament light mutants. PMID: 15857389
  6. 68-kDa neurofilament light chain (NF-L) as identified as a binding protein of amino-terminal PH domain of PLC-gamma1. PMID: 16819285
  7. Rat pituitary cells from the proopiomelanocortin lineage nearly completely lack NF68 immunoreactivity. PMID: 16940710
  8. Data show that as a consequence of RhoA knockdown, the process which expresses neurofilament is markedly induced in C12 cells. PMID: 18556119

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Subcellular Location
Cell projection, axon. Cytoplasm, cytoskeleton.
Protein Families
Intermediate filament family
Tissue Specificity
Expressed in the dorsal root ganglion neurons (at protein level).
Database Links
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