Rat OX-2 membrane glycoprotein(CD200) ELISA kit

Code CSB-EL004895RA
Size 96T,5×96T,10×96T
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Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
CD200 molecule
Alternative Names
Cd200; Mox2; OX-2 membrane glycoprotein; MRC OX-2 antigen; CD antigen CD200
Abbreviation
CD200
Uniprot No.
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates
Detection Range
47 pg/mL-3000 pg/mL
Sensitivity
11.75 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cardiovascular
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat CD200 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 84
Range % 80-88
1:2 Average % 90
Range % 86-94
1:4 Average % 95
Range % 90-100
1:8 Average % 92
Range % 88-96
Recovery
The recovery of rat CD200 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 106 102-113
EDTA plasma (n=4) 98 94-102
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
3000 2.271 2.317 2.294 2.103
1500 1.429 1.369 1.399 1.208
750 0.928 0.984 0.956 0.765
375 0.589 0.568 0.579 0.388
187.5 0.396 0.372 0.384 0.193
94 0.288 0.304 0.296 0.105
47 0.240 0.231 0.236 0.045
0 0.188 0.193 0.191  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Rat CD200 ELISA Kit was designed for the quantitative measurement of Rat CD200 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 47 pg/mL-3000 pg/mL and the sensitivity is 11.75 pg/mL.

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Target Background

Function
(From Uniprot)
Costimulates T-cell proliferation. May regulate myeloid cell activity in a variety of tissues.
Subcellular Location
Cell membrane; Single-pass type I membrane protein.
Tissue Specificity
Found on the surface of neurons, thymocytes, B-cells and follicular dendritic cells.
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