Rat OX-2 membrane glycoprotein(CD200) ELISA kit

Instructions
Code CSB-EL004895RA
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name CD200 molecule
Alternative Names Cd200 ELISA Kit; Mox2 ELISA Kit; OX-2 membrane glycoprotein ELISA Kit; MRC OX-2 antigen ELISA Kit; CD antigen CD200 ELISA Kit
Abbreviation CD200
Uniprot No. P04218
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 47 pg/mL-3000 pg/mL
Sensitivity 11.75 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat CD200 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 84
Range % 80-88
1:2 Average % 90
Range % 86-94
1:4 Average % 95
Range % 90-100
1:8 Average % 92
Range % 88-96
Recovery
The recovery of rat CD200 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 106 102-113
EDTA plasma (n=4) 98 94-102
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
3000 2.271 2.317 2.294 2.103
1500 1.429 1.369 1.399 1.208
750 0.928 0.984 0.956 0.765
375 0.589 0.568 0.579 0.388
187.5 0.396 0.372 0.384 0.193
94 0.288 0.304 0.296 0.105
47 0.240 0.231 0.236 0.045
0 0.188 0.193 0.191  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Data

Function Costimulates T-cell proliferation. May regulate myeloid cell activity in a variety of tissues.
Subcellular Location Cell membrane, Single-pass type I membrane protein
Tissue Specificity Found on the surface of neurons, thymocytes, B-cells and follicular dendritic cells.
Database Links

STRING: 10116.ENSRNOP00000055253

UniGene: Rn.7085

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