Rat Occludin (Ocln) ELISA kit

Code CSB-E17291r
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q6P6T5
Lead Time 3-5 working days
Abbreviation Ocln
Target Name Occludin (Ocln)
Alias , tight junction protein occludin TM4 minus
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 25 pg/mL-1600 pg/mL
Sensitivity 6.25 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This gene encodes an integral membrane protein which is located at tight junctions. This protein may be involved in the formation and maintenance of the tight junction. The possibility of several alternatively spliced products has been suggested but the full nature of these products has not been described.
HGNC 8104
RGD 620089
MGI 106183
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat Ocln in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 87
Range % 83-91
1:2 Average % 111
Range % 108-114
1:4 Average % 95
Range % 91-99
1:8 Average % 86
Range % 83-89
Recovery
The recovery of rat Ocln spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 99 95-104
EDTA plasma (n=4) 87 83-90
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
1600 2.886 2.801 2.844 2.701
800 2.404 2.357 2.381 2.238
400 1.788 1.804 1.796 1.653
200 1.263 1.302 1.283 1.140
100 0.677 0.701 0.689 0.546
50 0.452 0.431 0.442 0.299
25 0.251 0.263 0.257 0.114
0 0.141 0.145 0.143  
References
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Function May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. May be involved in the organization of actin in endothelial cells.
Subcellular Location Cell membrane, Multi-pass membrane protein, Cell junction, tight junction
Protein Families ELL/occludin family
Database Links

KEGG: rno:83497

STRING: 10116.ENSRNOP00000024674

UniGene: Rn.31429

Pathway Leukocyte transendothelial migration

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