Rat Occludin (Ocln) ELISA kit

Instructions
Code CSB-E17291r
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name Occludin (Ocln)
Alternative Names Ocln ELISA Kit; Occludin ELISA Kit
Abbreviation Ocln
Uniprot No. Q6P6T5
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 25 pg/mL-1600 pg/mL
Sensitivity 6.25 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat Ocln in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 87
Range % 83-91
1:2 Average % 111
Range % 108-114
1:4 Average % 95
Range % 91-99
1:8 Average % 86
Range % 83-89
Recovery
The recovery of rat Ocln spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 99 95-104
EDTA plasma (n=4) 87 83-90
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
1600 2.886 2.801 2.844 2.701
800 2.404 2.357 2.381 2.238
400 1.788 1.804 1.796 1.653
200 1.263 1.302 1.283 1.140
100 0.677 0.701 0.689 0.546
50 0.452 0.431 0.442 0.299
25 0.251 0.263 0.257 0.114
0 0.141 0.145 0.143  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. May be involved in the organization of actin in endothelial cells.
Gene References into Functions
  1. blood occludin levels correlate well with the extent of blood brain barrie damage. PMID: 28079139
  2. Both upregulation of claudin-4 and downregulation of occludin might increase paracellular NaCl transport in the kidney, resulting in impaired pressure natriuresis in SS rats. PMID: 29179201
  3. Up-regulation of miR-144 could promote intestinal hyperpermeability and impair the protective effect of the epithelial barrier by directly targeting OCLN and ZO-1. PMID: 29258088
  4. We provide quantitative evidence that claudin-11 contributes significantly to Sertoli cell tight junction function in vitro. Interestingly, occludin, which is hormonally regulated but not implicated in infertility until late adulthood, is also a significant contributor to barrier function. PMID: 26585695
  5. Mechanical ventilation can activate c-Src by phosphorylation and increase the degradation of occluding. PMID: 25471013
  6. In severe acute pancreatitis, downregulated expression of alphaSNAP in intestinal epithelial cells leads to reduced occludin expression, apoptosis of intestinal epithelial cells, and increased permeability of the intestinal barrier. PMID: 25278303
  7. actin cytoskeletal dynamics is detrimental to METH-induced BBB dysfunction by increasing internalization of occludin. PMID: 24081143
  8. cells transfected with Cx43 siRNA and grown in N medium showed significant downregulation in occludin (78 +/- 8% of control) and ZO-1 (81 +/- 6% of control) expression, and exhibited increased cell monolayer permeability. PMID: 24008412
  9. Results identified a crucial role of occludin in submandibular epithelial cells, and more importantly, demonstrated that occludin was required to mediate TRPV1-modulated paracellular permeability. PMID: 23345400
  10. High temperature inhibited the proliferation of rat Sertoli cells in vitro, and decreased the expression of OCLN. PMID: 23297502
  11. Disruption of pulmonary epithelium induced by hyperoxia is due in part to decreased expression of occludin and zona occludens (ZO)-1 proteins. PMID: 22559818
  12. Reverse-flow perfusions of mesenteric venules showed numerous small gaps in occludin expression on the endothelium. Similar gaps were not seen in vessels perfused in the forward direction. PMID: 23417864
  13. data indicate the possibility that interleukin 6 might be involved in the downregulation of occludin expression and in the modulation of blood-testis barrier permeability that occur in rats undergoing autoimmune orchitis PMID: 23018187
  14. Occludin and Zo-1 were reduced in intestinal mucosa both in mRNA and protein levels in the rat tail-suspension model. PMID: 21336719
  15. Occludin protein loss and claudin-5 redistribution are detected in ischemic microvessels following middle cerebral artery occlusion. PMID: 22378877
  16. Levels of tight junction protein occludin are decreased in blood-brain barrier cells post-translationally following exposure to neurotoxicants. PMID: 20970449
  17. A calcium-activated potassium channel activator increases blood-brain tumor-barrier permeability by down-regulating the expression of occludin. PMID: 21334421
  18. In female rats, increasing age and 17beta-estradiol treatment alters the expression of ERalpha and distinct blood-brain barrier tight-junction protein isoforms (occludin and claudin-5) without altering functional paracellular permeability. PMID: 21192956
  19. the evidence presented herein suggests the implication of occludin and, therefore, of blood-brain barrier in the pathophysiology of extrahepatic cholestasis. PMID: 20170644
  20. There was a significant down-regulation in the expression of occludin abd JAM-1 after exposure to microwave radiation. PMID: 20180397
  21. The presence of occludin in the tight junctions at the time of implantation suggests that the paracellular pathway is impermeable to water and Na(+) at this time, and that the transport of such substances takes place via the transcellular pathway. PMID: 19555995
  22. present in the junctions of the olfactory epithelium, and in the endothelial cells in the blood vessels in the lamina propria of the olfactory mucosa PMID: 11751462
  23. After ligation of the common bile duct, the expression of this protein was elevated in the liver. PMID: 11845325
  24. diabetes-related changes in cerebral zonula occludin-1 expression (zonula occludin-1) PMID: 11958524
  25. Occludin was immunoreactive at distal and proximal complexes of early differentiating ameloblasts and at distal regions of differentiating odontoblasts. However, in more advanced stages, occludin was only evident at the proximal complex of ameloblasts. PMID: 15052661
  26. In vitro blood-brain barrier model studies revealed that the pericyte-derived multimeric angiopoietin-1/Tie-2 pathway induces occludin expression PMID: 15056293
  27. Taken collectively, the results reported herein support the notion that db-cAMP-induced TJ disruption was mediated by an induction of Itch protein expression, which in turn triggered the ubiquitination of occludin resulting in TJ disruption. PMID: 15605377
  28. The effect of matrix metalloproteinase(MMP) inhibitors on endothelial gap formation, occludin loss, and the ability of monocytes to pass the endothelium is reported. PMID: 17065217
  29. These findings indicate the opposite effects of the NMDA and AMPA/kainate receptors on occludin phosphorylation and disruption of the blood-brain barrier. PMID: 17245419
  30. Immunohistochemical expression of occludin and connexin 43 were decreased in the testis after vasal and epididymal ligation. PMID: 17825302
  31. The increase of BK-mediated BTB permeability is associated with the down-regulation of ZO-1, occludin, and claudin-5 and the rearrangement of F-actin;cAMP/PKA might be involved in the modulating process. PMID: 18183615
  32. Chronic ethanol ingestion impairs the airway epithelial barrier function through down-regulation and disruption of membrane localization of occludin and E-cadherin. PMID: 18279593
  33. Increased BBB permeability associated with peripheral inflammatory pain is promoted by the disruption of disulfide-bonded occludin oligomeric assemblies, which renders them incapable of forming an impermeant physical barrier to paracellular transport. PMID: 18647175
  34. The changes in occludin distribution and decreased expression of ZO-1 lead the reorganization of BBB-actin protein, which may be one of the mechanisms of permeability increasing of BBB following hypoxia-ischemia. PMID: 18706176
  35. Cardiopulmonary bypass markedly down-regulates the expression of occludin and ZO-1 proteins in intestinal mucosa of rats. PMID: 18855986
  36. a unique motif in the occludin sequence that is involved in the regulation of ZO-1 binding by reversible phosphorylation of specific Tyr residues. PMID: 19017651
  37. Transient focal ischemia increased the tyrosine phosphorylation of occludin in the isolated brain capillaries PMID: 19319148
  38. Occludin performs a structural role in inhibiting paracellular diffusion, and a signaling role involving interactions of dimeric and monomeric occludin isoforms within plasma membrane lipid raft domains. PMID: 19457074
  39. Fg binding to endothelial cells (ECs) alters expression of actin-associated endothelial tight junction proteins. PMID: 19507189

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Subcellular Location Cell membrane, Multi-pass membrane protein, Cell junction, tight junction
Protein Families ELL/occludin family
Database Links

KEGG: rno:83497

STRING: 10116.ENSRNOP00000024674

UniGene: Rn.31429

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