Rat Platelet Factor 4,PF-4 ELISA Kit

Code CSB-E07885r
Size 96T,5×96T,10×96T
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Product Details

Target Name
platelet factor 4
Alternative Names
Pf4 ELISA Kit; Cxcl4 ELISA Kit; Scyb4 ELISA Kit; Platelet factor 4 ELISA Kit; PF-4 ELISA Kit; C-X-C motif chemokine 4 ELISA Kit
Abbreviation
PF4
Uniprot No.
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.156 μg/mL-10 μg/mL
Sensitivity
0.039 μg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cardiovascular
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat PF-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %90
Range %86-97
1:2Average %95
Range %88-99
1:4Average %95
Range %91-102
1:8Average %95
Range %87-104
Recovery
The recovery of rat PF-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9583-99
EDTA plasma (n=4)9789-102
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
μg/mlOD1OD2AverageCorrected
102.684 2.617 2.651 2.463
52.204 2.186 2.195 2.007
2.51.563 1.608 1.586 1.398
1.250.881 0.899 0.890 0.702
0.6250.480 0.491 0.486 0.298
0.3120.345 0.337 0.341 0.153
0.1560.247 0.250 0.249 0.061
00.185 0.190 0.188
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Rat PF4 ELISA Kit was designed for the quantitative measurement of Rat PF4 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 μg/mL-10 μg/mL and the sensitivity is 0.039 μg/mL.

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Target Background

Function
(From Uniprot)
Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation.
Gene References into Functions
  1. identified the informative Chronic Liver Allograft Dysfunction (CLAD)-associated genes; CXCL4, CXCR3, EGFR, JAK2, STAT3, and Collagen IV were associated with CLAD pathogenesis; validated that CXCL4 is upstream of these informative genes in the isolated hepatic stellate cells PMID: 28053995
  2. A carboxyl-terminal fragment of this protein effects a rapid histamine release from rat peritoneal mast cells. PMID: 12383857
  3. this study identified a novel regulatory element,TME(tandem repeat of MEIS1 binding element)in the PF4 promoter and demonstrated that MEIS1/PBX complexes bind to the TME PMID: 12609849
  4. PF4 promoters are transactivated by USF1 and 2 which may play an important role in megakaryocytic gene expression PMID: 15187018
  5. The levels of PF-4 (as a platelet marker) mRNA were significantly higher in FeCl(2)-VT (2(-DeltaDeltaCT)=7.8) than in stasis-VT (2(-DeltaDeltaCT)=4.2, p<0.05). PMID: 16797058

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Subcellular Location
Secreted.
Protein Families
Intercrine alpha (chemokine CxC) family
Database Links
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