Rat Sclerostin(SOST) ELISA kit

Code CSB-EL022415RA
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q99P67
Lead Time 5-7 working days
Abbreviation SOST
Protein Biological Process 1 Signaling Pathway
Target Name sclerosteosis
Alias VBCH, sclerostin
Species Rattus norvegicus (Rat)
Protein Biological Process 3 Wnt signaling pathway
Sample Types serum, plasma, tissue homogenates
Detection Range 18.75 pg/mL-1200 pg/mL
Sensitivity 4.68 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details Sclerostin is a secreted glycoprotein with a C-terminal cysteine knot-like (CTCK) domain and sequence similarity to the DAN (differential screening-selected gene aberrative in neuroblastoma) family of bone morphogenetic protein (BMP) antagonists. Loss-of-function mutations in this gene are associated with an autosomal-recessive disorder, sclerosteosis, which causes progressive bone overgrowth. A deletion downstream of this gene, which causes reduced sclerostin expression, is associated with a milder form of the disorder called van Buchem disease.
HGNC 13771
RGD 69358
MGI 1921749
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat SOST in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %97
Range %93-101
1:2Average %92
Range %86-99
1:4Average %90
Range %88-94
1:8Average %101
Range %96-103
Recovery
The recovery of rat SOST spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9794-103
EDTA plasma (n=4)105105-109
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
12002.362 2.545 2.454 2.316
6001.588 1.686 1.637 1.499
3001.123 1.102 1.113 0.975
1500.767 0.744 0.756 0.618
750.520 0.546 0.533 0.395
37.50.304 0.324 0.314 0.176
18.750.248 0.241 0.245 0.107
00.136 0.140 0.138
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Function Negative regulator of bone growth that acts through inhibition of Wnt signaling and bone formation.
Subcellular Location Secreted
Protein Families Sclerostin family
Database Links

KEGG: rno:80722

STRING: 10116.ENSRNOP00000028238

UniGene: Rn.95369

Pathway Wnt signaling pathway

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