Rat Soluble Lectin-like Oxidized Low Density Lipoprotein Receptor-1(sLOX-1)ELISA Kit

Code CSB-E15845r
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. O70156
Lead Time 3-5 working days
Abbreviation OLR1
Protein Biological Process 1 Cell Adhesion
Target Name oxidized low density lipoprotein (lectin-like) receptor 1
Alias CLEC8A, LOX1, SCARE1, lectin-type oxidized LDL receptor 1|oxidised low density lipoprotein (lectin-like) receptor 1|scavenger receptor class E, member 1
Species Rattus norvegicus (Rat)
Protein Biological Process 3 Cell adhesion
Sample Types serum, plasma, tissue homogenates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This gene encodes a receptor protein which belongs to the C-type lectin superfamily. This gene is regulated through the cyclic AMP signaling pathway. The encoded protein binds, internalizes and degrades oxidized low-density lipoprotein. This protein may be involved in the regulation of Fas-induced apoptosis. This protein may play a role as a scavenger receptor. Mutations of this gene have been associated with atherosclerosis, risk of myocardial infarction, and may modify the risk of Alzheimer s disease. Alternative transcript variants have been described but their full-length sequence has not been determined.
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat sLOX-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %90
Range %85-94
1:2Average %92
Range %88-99
1:4Average %88
Range %84-92
1:8Average %95
Range %90-100
Recovery
The recovery of rat sLOX-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8580-90
EDTA plasma (n=4)9490-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1OD2AverageCorrected
102.375 2.227 2.301 2.190
51.816 1.902 1.859 1.748
2.51.240 1.208 1.224 1.113
1.250.725 0.785 0.755 0.644
0.6250.421 0.439 0.430 0.319
0.3120.263 0.284 0.274 0.163
0.1560.218 0.221 0.220 0.109
00.109 0.112 0.111
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Function Receptor that mediates the recognition, internalization and degradation of oxidatively modified low density lipoprotein (oxLDL) by vascular endothelial cells. OxLDL is a marker of atherosclerosis that induces vascular endothelial cell activation and dysfunction, resulting in pro-inflammatory responses, pro-oxidative conditions and apoptosis. Its association with oxLDL induces the activation of NF-kappa-B through an increased production of intracellular reactive oxygen and a variety of pro-atherogenic cellular responses including a reduction of nitric oxide (NO) release, monocyte adhesion and apoptosis. In addition to binding oxLDL, it acts as a receptor for the HSP70 protein involved in antigen cross-presentation to naive T-cells in dendritic cells, thereby participating in cell-mediated antigen cross-presentation. Also involved in inflammatory process, by acting as a leukocyte-adhesion molecule at the vascular interface in endotoxin-induced inflammation. Also acts as a receptor for advanced glycation end (AGE) products, activated platelets, monocytes, apoptotic cells and both Gram-negative and Gram-positive bacteria.
Subcellular Location Cell membrane, Lipid-anchor, Cell membrane, Single-pass type II membrane protein, Membrane raft, Secreted
Tissue Specificity Predominantly expressed in lung and at lower level in kidney. Expressed in macrophages but not in vascular smooth muscle cells.
Database Links

KEGG: rno:140914

STRING: 10116.ENSRNOP00000011196

UniGene: Rn.87449

Pathway PPAR signaling pathway

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