Rat Translationally-controlled tumor protein(TPT1) ELISA kit

Code CSB-EL024134RA
Size 96T,5×96T,10×96T
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Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
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Product Details

Target Name
tumor protein, translationally-controlled 1
Alternative Names
Tpt1 ELISA Kit; Trt ELISA Kit; Translationally-controlled tumor protein ELISA Kit; TCTP ELISA Kit; Lens epithelial protein ELISA Kit
Abbreviation
TPT1
Uniprot No.
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates, cell lysates
Detection Range
39 pg/mL-2500 pg/mL
Sensitivity
9.75 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat TPT1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %84
Range %80-88
1:2Average %94
Range %90-98
1:4Average %98
Range %94-104
1:8Average %95
Range %88-99
Recovery
The recovery of rat TPT1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9889-104
EDTA plasma (n=4)9490-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
25002.047 2.134 2.091 1.959
12501.384 1.483 1.434 1.302
6250.854 0.836 0.845 0.713
312.50.517 0.524 0.521 0.389
1560.337 0.346 0.342 0.210
780.257 0.248 0.253 0.121
390.195 0.201 0.198 0.066
00.134 0.129 0.132  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Rat TPT1 ELISA Kit was designed for the quantitative measurement of Rat TPT1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 39 pg/mL-2500 pg/mL and the sensitivity is 9.75 pg/mL .

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Target Background

Function
(From Uniprot)
Involved in calcium binding and microtubule stabilization.
Gene References into Functions
  1. Tumor protein translationally-controlled -induced dopamine release from neuronal PC12 cells was modulated by a Ca(2+)-independent mechanism that involved PLA in the process, suggesting the regulatory role of TCTP in the neuronal functions. PMID: 27783042
  2. After acute spinal cord injury, TCTP in astrocytes gradually increased, peaking at day 3 and returning to basal levels by day 14. TCTP knockdown reduced astrocyte migration and proliferation. PMID: 26266488
Subcellular Location
Cytoplasm.
Protein Families
TCTP family
Database Links
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