Rat Tripartite motif-containing protein 72(TRIM72) ELISA kit

Code CSB-EL024511RA
Size 96T,5×96T,10×96T
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Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
tripartite motif-containing 72
Alternative Names
Trim72 ELISA Kit; Mg53 ELISA Kit; Tripartite motif-containing protein 72 ELISA Kit; Mitsugumin-53 ELISA Kit; Mg53 ELISA Kit
Uniprot No.
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates, cell lysates
Detection Range
18.75 pg/mL-1200 pg/mL
4.7 pg/mL
Assay Time
Sample Volume
Detection Wavelength
450 nm
Research Area
Cell Biology
Assay Principle
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of rat TRIM72 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:1Average %86
Range %80-92
1:2Average %97
Range %91-105
1:4Average %99
Range %92-110
1:8Average %93
Range %86-98
The recovery of rat TRIM72 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9489-98
EDTA plasma (n=4)9690-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
12002.814 2.989 2.902 2.817
6002.366 2.441 2.404 2.319
3001.559 1.457 1.508 1.423
1500.753 0.697 0.725 0.640
750.469 0.442 0.456 0.371
37.50.243 0.235 0.239 0.154
18.750.149 0.139 0.144 0.059
00.088 0.082 0.085  
and FAQs
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

This Rat TRIM72 ELISA Kit was designed for the quantitative measurement of Rat TRIM72 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 18.75 pg/mL-1200 pg/mL and the sensitivity is 4.7 pg/mL.

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Target Background

(From Uniprot)
Muscle-specific protein that plays a central role in cell membrane repair by nucleating the assembly of the repair machinery at injury sites. Specifically binds phosphatidylserine. Acts as a sensor of oxidation: upon membrane damage, entry of extracellular oxidative environment results in disulfide bond formation and homooligomerization at the injury site. This oligomerization acts as a nucleation site for recruitment of TRIM72-containing vesicles to the injury site, leading to membrane patch formation. Probably acts upstream of the Ca(2+)-dependent membrane resealing process. Required for transport of DYSF to sites of cell injury during repair patch formation. Regulates membrane budding and exocytosis. May be involved in the regulation of the mobility of KCNB1-containing endocytic vesicles.
Gene References into Functions
  1. These results suggest that an 8-week swimming exercise improves HFD-induced insulin resistance maybe through a reduction of TRIM72 in skeletal muscle and enhancement of AKT signal transduction. PMID: 27843952
  2. MG53 is an effective biomarker of myocardial injury and dysfunction in murine hearts. However, MG53 is not expressed in human heart and therefore does not hold utility as a clinical biomarker of myocardial injury PMID: 26790476
  3. MG53 is a facilitator of rapid injury repair, a mediator of cell migration, and a modulator of myofibroblast differentiation during wound healing PMID: 26306047
  4. MG53, a member of the muscle-specific transmembrane adaptor molecule (TRIM) protein family, is a crucial component of the cardiac ischemic preconditioning response. PMID: 20516375
Subcellular Location
Cell membrane, sarcolemma. Cytoplasmic vesicle membrane.
Protein Families
TRIM/RBCC family
Database Links
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