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Rat Troponin I, slow skeletal muscle(TNNI1) ELISA kit

Citations (1) Protocol
Code CSB-EL024011RA
Size 96T,5×96T,10×96T
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* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
troponin I type 1 (skeletal, slow)
Alternative Names
Tnni1 ELISA Kit; Troponin I ELISA Kit; slow skeletal muscle ELISA Kit; Troponin I ELISA Kit; slow-twitch isoform ELISA Kit
Abbreviation
TNNI1
Uniprot No.
P13413
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates
Detection Range
15.6 pg/mL-1000 pg/mL
Sensitivity
3.9 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat TNNI1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
　 Sample Serum(n=4)    
1:1 Average % 84    
Range % 80-88    
1:2 Average % 90    
Range % 86-94    
1:4 Average % 95    
Range % 90-100    
1:8 Average % 92    
Range % 88-96    
Recovery
The recovery of rat TNNI1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 88 84-92    
EDTA plasma (n=4) 103 99-108    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
pg/ml OD1 OD2 Average Corrected    
1000 2.526 2.423 2.475 2.289    
500 1.875 1.756 1.816 1.630    
250 1.212 1.159 1.186 1.000    
125 0.784 0.811 0.798 0.612    
62.5 0.500 0.490 0.495 0.309    
31.2 0.347 0.362 0.355 0.169    
15.6 0.291 0.274 0.283 0.097    
0 0.183 0.188 0.186 　    
ELISA Data Analysis
ELISA Standard Curve & Curve Expert software
Troubleshooting
and FAQs
ELISA kit FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Rat TNNI1 ELISA Kit was designed for the quantitative measurement of Rat TNNI1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

Citations

Related Products

Tnni1 Proteins

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Target Background

Function
(From Uniprot)
Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
Gene References into Functions
  1. these results suggest that cTnI(R146G) and cTnI(R21C) blunt PKA modulation of activation and relaxation kinetics by prohibiting cardiac-specific N-terminal interaction with the cTnI inhibitory peptide. PMID: 26391394
  2. troponin I phosphorylation has a role in protein kinase C-mediated enhanced contractile performance of myocytes PMID: 12815045
  3. A novel role is described for cardiac TnI PKA phosphorylation in the rate-dependent enhancement of systolic & diastolic function in vivo & afterload sensitivity of relaxation. This has implications for cardiac failure. PMID: 14726477
  4. MuRF1 functions as a ubiquitin ligase to catalyze ubiquitylation of troponin I through a RING finger-dependent mechanism PMID: 15601779
  5. TnI-isoform and titin-isoform switching and corresponding functional changes during heart development are not initiated by birth but are genetically programmed PMID: 16679402
  6. The goal here was to identify domain clusters and residues that confer troponin I isoform-specific myofilament Ca2+ and pH sensitivities of contraction. PMID: 17602701
  7. This study highlights cellular contractile defects by cardiomyopathy mutant cTnIs that are allele and species dependent. PMID: 18423659
  8. Study identifies tyrosine phosphorylation as a novel protein kinase Cdelta (PKCdelta) activation mechanism that modifies PKCdelta-dependent phosphorylation of cardiac troponin I. PMID: 18550549
  9. The negative inotropic effect of HIV gp120 is mediated by a novel signaling pathway that begins with binding to a cardiac myocyte CXCR4 receptor, followed by phosphorylation of both p38 MAP kinase and Troponin I. PMID: 18850076
  10. observed higher relative level of natural antisense RNA in neonatal cardiac fibroblasts compared to neonatal cardiomyocytes, pointing to a cell type-specific expression of sense and antisense RNA PMID: 19184367
  11. These findings suggest that altered TnI function, due to changes in the distribution of phosphorylated sites, is an early contributor to reduced contractility of the heart. PMID: 19507043

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