Rat Tumor necrosis factor soluble receptor Ⅱ,TNFsR-Ⅱ ELISA KIT

Instructions
Code CSB-E07380r
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name Tumor necrosis factor soluble receptor Ⅱ,TNFsR-
Alternative Names Tnfrsf1b ELISA Kit; Tnfr2 ELISA Kit; Tumor necrosis factor receptor superfamily member 1B ELISA Kit; Tumor necrosis factor receptor 2 ELISA Kit; TNF-R2 ELISA Kit; Tumor necrosis factor receptor type II ELISA Kit; TNF-RII ELISA Kit; TNFR-II ELISA Kit; p75 ELISA Kit; p80 TNF-alpha receptor ELISA Kit; CD antigen CD120b ELISA Kit
Abbreviation TNFsR2
Uniprot No. Q80WY6
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.625 ng/mL-40 ng/mL
Sensitivity 0.156 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat TNFsR-II in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 91
Range % 87-94
1:2 Average % 97
Range % 92-101
1:4 Average % 90
Range % 87-98
1:8 Average % 92
Range % 85-95
Recovery
The recovery of rat TNFsR-II spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 93 88-96
EDTA plasma (n=4) 98 95-102
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
40 2.827 2.899 2.863 2.732
20 2.222 2.323 2.273 2.142
10 1.373 1.412 1.393 1.262
5 0.816 0.866 0.841 0.710
2.5 0.531 0.545 0.538 0.407
1.25 0.315 0.323 0.319 0.188
0.625 0.226 0.241 0.234 0.103
0 0.126 0.135 0.131  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Target Data

Function Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2 (By similarity).
Gene References into Functions
  1. results suggested that PTE may promote BMSC proliferation and osteogenic differentiation via a mechanism associated with the regulation of Let-7f-5p and the TNFR2/PI3K/AKT signaling pathway. PMID: 29975930
  2. Curcumin decreased TNFR2 and increased TRAF2 to decrease the apoptotic pathway in ureteral obstruction rats. PMID: 26732833
  3. The IL-17RD.TNFR2 complex in activation of NF-kappaB may explain the role of TNFR2 in inflammatory diseases including nephritis. PMID: 25378394
  4. the microglial TNF-alpha p55 pathway played a more important role than the TNF-alpha p75 pathway in the pathogenesis of peripheral nerve injury pain. PMID: 22652595
  5. study of TNF-alpha and TnfrR2 protein synthesis and expression patterns in sciatic nerve of controls and rats under systemic challenge with lipopolysaccharide. PMID: 17873366
  6. In human biopsies and in rat kidney allografts on day 6 with acute allograft rejection, significantly elevated expression of TNFR2 was observed. PMID: 19298452

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Subcellular Location Membrane, Single-pass type I membrane protein
Database Links

KEGG: rno:156767

STRING: 10116.ENSRNOP00000022478

UniGene: Rn.83633

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