Product Details

Target Name

VGF Nerve Growth Factor Inducible (VGF)

Alternative Names

VgfNeurosecretory protein VGF ELISA kit; VGF8a protein) [Cleaved into: VGF(24-63); VGF(180-194); VGF(375-407); Neuroendocrine regulatory peptide-1 ELISA kit; NERP-1); Neuroendocrine regulatory peptide-2 ELISA kit; NERP-2); VGF-derived peptide TLQP-11; VGF-derived peptide TLQP-21; VGF-derived peptide TLQP-30; VGF-derived peptide TLQP-62; VGF-derived peptide HFHH-10; VGF-derived peptide AQEE-30; VGF-derived peptide LQEQ-19] ELISA kit

Abbreviation

VGF

Uniprot No.

P20156

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma

Detection Range

0.312 ng/mL-20 ng/mL

Sensitivity

0.078 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Neuroscience

Assay Principle

quantitative

Measurement

Sandwich

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

The Rat VGF Nerve Growth Factor Inducible (VGF) ELISA Kit is a powerful tool for researchers in the field of neuroscience. This quantitative assay measures the concentration of VGF Nerve Growth Factor Inducible (VGF) in serum and plasma samples from Rattus norvegicus (Rat).

With a detection range of 0.312 ng/mL to 20 ng/mL and a sensitivity of 0.078 ng/mL, this ELISA kit provides accurate and precise measurements for your research needs. The assay time ranges from 1 to 5 hours, and the sample volume required is 50-100ul.

The sandwich measurement technique used in this kit ensures high specificity and sensitivity, providing you with reliable and consistent results. The detection wavelength of 450 nm ensures accurate measurements for each sample.

This ELISA kit is particularly useful for researchers in the field of neuroscience, as VGF has been linked to neuronal development and plasticity, as well as cognitive function and behavior. This kit can help investigate the role of VGF in these processes and provide insights into potential therapeutic targets.

Target Background

Function
(From Uniprot)

Secreted polyprotein that is packaged and proteolytically processed by prohormone convertases PCSK1 and PCSK2 in a cell-type-specific manner. VGF and peptides derived from its processing play many roles in neurogenesis and neuroplasticity associated with learning, memory, depression and chronic pain.; Plays a role in the control of body fluid homeostasis by regulating vasopressin release. Suppresses presynaptic glutamatergic neurons connected to vasopressin neurons.; Plays a role in the control of body fluid homeostasis by regulating vasopressin release. Activates GABAergic interneurons which are inhibitory neurons of the nervous system and thereby suppresses presynaptic glutamatergic neurons. Stimulates also feeding behavior in an orexin-dependent manner in the hypothalamus. Functions as a positive regulator for the activation of orexin neurons resulting in elevated gastric acid secretion and gastric emptying.; Secreted multifunctional neuropeptide that binds to different cell receptors and thereby plays multiple physiological roles including modulation of energy expenditure, pain, response to stress, gastric regulation, glucose homeostasis as well as lipolysis. Activates the G-protein-coupled receptor C3AR1 via a folding-upon-binding mechanism leading to enhanced lipolysis in adipocytes. Interacts with C1QBP receptor in macrophages and microglia causing increased levels of intracellular calcium and hypersensitivity.; Plays a role in the regulation of memory formation and depression-related behaviors potentially by influencing synaptic plasticity and neurogenesis. Induces acute and transient activation of the NTRK2/TRKB receptor and subsequent CREB phosphorylation. Induces also insulin secretion in insulinoma cells by increasing intracellular calcium mobilization.

Gene References into Functions

Results indicate that neuron-restrictive silencer factor plays an important role as a repressor of VGF gene regulation in neuroblastoma cells through a mechanism that is dependent on VGF-neuron-restrictive silencer element PMID: 25569790
various VGF peptides may regulate the hypothalamus-pituitary complex via specific neuroendocrine mechanisms while TLQP peptides may act at further, multiple levels via endocrine mechanisms involving the ovary. PMID: 25280008
VGF is involved in the control of metabolism and body weight homeostasis; active peptide fragments are generated from VGF, including TLQP-21, which controls reproductive function in female rats. PMID: 23485923
These results indicate that gC1qR is the receptor for TLQP-21 and plays an important role in chronic pain through activation of macrophages PMID: 24106277
VGF expression under continuous stress is negatively regulated by dopaminergic neurons projecting from the hypothalamus. PMID: 23541636
Specific VGF peptides appear to be widely represented in different gastric endocrine and other mucosal cell populations. PMID: 20876237
VGF protein is primarily cleaved at 10 sites through the processing pathway common to the brain and gastrointestinal tract. PMID: 20524965
Analysis of the contractile activity of VGF-derived peptides, biologically active in other systems, shows that only TLQP-21, corresponding to the 556-576 amino acid fragment of VGF precursor protein, can influence gastrointestinal contraction in vitro. PMID: 19466987
VGF neuropeptides may regulate synaptic function in hippocampal neurons; C-terminal VGF peptides acutely increased synaptic charge in a dose-dependent manner PMID: 14645472
molecular mechanisms of its sorting to the regulated and polarized pathways of secretion PMID: 15051509
The identification in the Central nervous system of a previously uncharacterized VGF-derived peptide and its chronic i.c.v. infusion effected an increase in energy expenditure and limited the early phase of diet-induced obesity, is reported. PMID: 16983076
the exercise-regulated gene VGF has an antidepressant effect PMID: 18059283
Prolonged upregulation of VGF mRNA and protein was observed in injured dorsal root ganglion neurons, central terminals and their target dorsal horn neurons PMID: 19077191

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Subcellular Location

[Neurosecretory protein VGF]: Secreted. Cytoplasmic vesicle, secretory vesicle.

Tissue Specificity

Central and peripheral nervous systems, synthesized exclusively in neuronal and neuroendocrine cells. VGF and several of the derived peptides are present in the brain.

Database Links

KEGG: rno:29461

STRING: 10116.ENSRNOP00000050024

UniGene: Rn.9704

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Code	CSB-E17520r
Size	96T,5×96T,10×96T
Price	Request a Quote or Start an on-line Chat
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Rat VGF Nerve Growth Factor Inducible (VGF) ELISA Kit

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