Rat Vascular Endothelial cell Growth Factor D,VEGF-D ELISA kit

Instructions
Code CSB-E07352r
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name c-fos induced growth factor (vascular endothelial growth factor D)
Alternative Names Vegfd ELISA kit; FigfVascular endothelial growth factor D ELISA kit; VEGF-D ELISA kit; c-Fos-induced growth factor ELISA kit; FIGF ELISA kit
Abbreviation Vegfd
Uniprot No. O35251
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat VEGF-D in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 87
Range % 83-92
1:2 Average % 101
Range % 97-105
1:4 Average % 91
Range % 85-97
1:8 Average % 97
Range % 91-103
Recovery
The recovery of rat VEGF-D spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 91 86-95
EDTA plasma (n=4) 98 94-102
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
20 1.932 1.837 1.885 1.738
10 1.456 1.566 1.511 1.364
5 1.088 1.042 1.065 0.918
2.5 0.713 0.707 0.710 0.563
1.25 0.492 0.481 0.487 0.340
0.625 0.358 0.342 0.350 0.203
0.312 0.272 0.278 0.275 0.128
0 0.148 0.146 0.147  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function Growth factor active in angiogenesis, lymphangiogenesis and endothelial cell growth, stimulating their proliferation and migration and also has effects on the permeability of blood vessels. May function in the formation of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. Binds and activates VEGFR-3 (Flt4) receptor (By similarity).
Gene References into Functions
  1. Expression of VEGF-D and its receptor, VEGFR-3, are enhanced in various cell types in the infarcted heart. PMID: 22818386
  2. Adenovirus encoding vascular endothelial growth factor-D induces tissue-specific vascular patterns in vivo. PMID: 12036873
  3. Overexpression of VEGF-D is associated with Gliosarcomas PMID: 16533777
  4. VEGF-D expression may have a role in interleukin (IL)-6 expression and transcription factor AP-1 activation in pancreatic acinar cells. PMID: 17404025
Subcellular Location Secreted
Protein Families PDGF/VEGF growth factor family
Tissue Specificity Highly expressed in the spleen, kidney, lung, tongue, ovary and mammary gland.
Database Links

KEGG: rno:360457

STRING: 10116.ENSRNOP00000058883

UniGene: Rn.10796

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