Rat Vascular Endothelial cell Growth Factor D,VEGF-D ELISA kit

Code CSB-E07352r
Size 96T,5×96T,10×96T
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Product Details

Target Name
c-fos induced growth factor (vascular endothelial growth factor D)
Alternative Names
Vegfd ELISA kit; FigfVascular endothelial growth factor D ELISA kit; VEGF-D ELISA kit; c-Fos-induced growth factor ELISA kit; FIGF ELISA kit
Abbreviation
Vegfd
Uniprot No.
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.312 ng/mL-20 ng/mL
Sensitivity
0.078 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cardiovascular
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat VEGF-D in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 87
Range % 83-92
1:2 Average % 101
Range % 97-105
1:4 Average % 91
Range % 85-97
1:8 Average % 97
Range % 91-103
Recovery
The recovery of rat VEGF-D spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 91 86-95
EDTA plasma (n=4) 98 94-102
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
20 1.932 1.837 1.885 1.738
10 1.456 1.566 1.511 1.364
5 1.088 1.042 1.065 0.918
2.5 0.713 0.707 0.710 0.563
1.25 0.492 0.481 0.487 0.340
0.625 0.358 0.342 0.350 0.203
0.312 0.272 0.278 0.275 0.128
0 0.148 0.146 0.147  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

The Rat Vascular Endothelial cell Growth Factor D (VEGF-D) ELISA kit is a powerful tool for researchers studying the cardiovascular system in rats. VEGF-D is a protein known for its role in promoting the growth and survival of blood vessels, making it an important target for cardiovascular research.

Our ELISA kit utilizes a quantitative sandwich assay principle to measure the levels of VEGF-D in serum, plasma, and tissue homogenates with a detection range of 0.312 ng/mL to 20 ng/mL and a sensitivity of 0.078 ng/mL. With an assay time of only 1-5 hours and a sample volume of 50-100ul, this kit is both efficient and convenient for researchers.

Whether you are investigating the mechanisms of angiogenesis and lymphatic regeneration or studying the effects of cardiovascular disease on the vasculature, this Rat Vascular Endothelial cell Growth Factor D (VEGF-D) ELISA kit is the perfect tool for your research needs.

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Target Background

Function
(From Uniprot)
Growth factor active in angiogenesis, lymphangiogenesis and endothelial cell growth, stimulating their proliferation and migration and also has effects on the permeability of blood vessels. May function in the formation of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. Binds and activates VEGFR-3 (Flt4) receptor.
Gene References into Functions
  1. Expression of VEGF-D and its receptor, VEGFR-3, are enhanced in various cell types in the infarcted heart. PMID: 22818386
  2. Adenovirus encoding vascular endothelial growth factor-D induces tissue-specific vascular patterns in vivo. PMID: 12036873
  3. Overexpression of VEGF-D is associated with Gliosarcomas PMID: 16533777
  4. VEGF-D expression may have a role in interleukin (IL)-6 expression and transcription factor AP-1 activation in pancreatic acinar cells. PMID: 17404025
Subcellular Location
Secreted.
Protein Families
PDGF/VEGF growth factor family
Tissue Specificity
Highly expressed in the spleen, kidney, lung, tongue, ovary and mammary gland.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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