Rat aldehyde dehydrogenase,ALDH ELISA Kit

Instructions
Code CSB-E11179r
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name aldehyde dehydrogenase 2 family (mitochondrial)
Alternative Names Aldh2 ELISA Kit; Aldehyde dehydrogenase ELISA Kit; mitochondrial ELISA Kit; EC 1.2.1.3 ELISA Kit; ALDH class 2 ELISA Kit; ALDH-E2 ELISA Kit; ALDH1 ELISA Kit
Abbreviation ALDH2
Uniprot No. P11884
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat ALDH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 100  
Range % 91-105  
1:2 Average % 98  
Range % 94-103  
1:4 Average % 96  
Range % 90-100  
1:8 Average % 98  
Range % 95-102  
Recovery
The recovery of rat ALDH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 90 86-95  
EDTA plasma (n=4) 91 87-96  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
20 2.764 2.753 2.759 2.560  
10 2.159 2.042 2.101 1.902  
5 1.529 1.512 1.521 1.322  
2.5 1.045 1.018 1.032 0.833  
1.25 0.682 0.656 0.669 0.470  
0.625 0.423 0.410 0.417 0.218  
0.312 0.312 0.303 0.308 0.109  
0 0.201 0.197 0.199    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Gene References into Functions
  1. ALDH2 inhibition protects against hepatic ischemia-reperfusion injury in rats via alleviation of oxidative stress. PMID: 29589772
  2. findings suggested that ALDH2 is expressed in neonate rat cardiac fibroblasts; activation of ALDH2 decreases the HG-induced apoptosis and fibrosis through inhibition of oxidative stress. PMID: 29129988
  3. ALDH2 O-GlcNAc modification is associated with hyperglycemic exacerbation during myocardial ischemia/reperfusion injury. PMID: 28038474
  4. Chronic hyperglycemia reduces ALDH2 activity, leading to mitochondrial respiratory dysfunction and consequently cardiac damage and dysfunction. PMID: 27736868
  5. Mitochondrial aldehyde dehydrogenase activation by alda-1 led to a significant attenuation of depressive- and anxiety-like behaviors in the prenatally stressed rats. PMID: 26254233
  6. High concentrations of ethanol aggravate cerebral injury by inhibiting of aldehyde dehydrogenase 2 and inducing excess accumulation of aldehydes PMID: 26172086
  7. The administration of the ALDH2-coding vector to the ventral tegmental area leading to an increased ability to degrade acetaldehyde greatly reduces chronic alcohol consumption and post-deprivation binge drinking for prolonged periods. PMID: 24571199
  8. Aberrant hypermethylation of CpG sites in ALDH2 promoter upstream sequence is associated with myocardial ischemia injury. PMID: 25629048
  9. ALDH2 is inhibition of MPTP opening and iNOS expression in cardiac function in response to treatment with Alda1, a specific activator of ALDH2. PMID: 25351957
  10. ALDH2 may be at least in part involved in nitrite-mediated hypotensive effects and nitrite catalysis in many organs of normoxic rats. PMID: 24164360
  11. Selective activation of mitochondrial aldehyde dehydrogenase 2 is sufficient to improve the heart failure outcome PMID: 24817685
  12. the mitochondrial enzyme ALDH2 regulates nociception PMID: 25163478
  13. Ethanol was used induce ALDH2 activity, which decreased myocardial injury in diabetic rats. PMID: 23500772
  14. In diabetic rats, enhanced ALDH2 expression can offer mycardial protection possibly by suppressing cell apoptosis. PMID: 22445980
  15. In diabetic rats, the expression of ALDH2 was decreased when the heart was subjected to ischemia/reperfusion. PMID: 22737913
  16. ALDH2 activation substantially reduces hypoxia-induced norepinephrine release during myocardial ischemia. PMID: 22761303
  17. The protective role of ALDH2 against ER stress-induced cell death was probably mediated by Akt via a p47(phox) NADPH oxidase-dependent manner. These findings indicate the critical role of ALDH2 in the pathogenesis of ER stress in heart disease. PMID: 22430940
  18. Changes in the GSH and GSH enzymatic system, and in the ADH3 and ALDH2 retinal expression and distribution might be crucial in assessing the intrinsic mechanisms of LPO-mediated retinopathies. PMID: 22117533
  19. study provides evidence that the simultaneous increase of liver ADH and a reduction of ALDH activity by gene transfer could constitute a potential therapeutic strategy for the treatment of alcoholism. PMID: 22214999
  20. our results suggested that ALDH2 might play a vital role in protecting antimycin A-induced oxidative stress and apoptosis in cardiomyocytes PMID: 21123025
  21. A decrease was observed in both ALDH2 protein expression (80% in tolerant veins and 30% in tolerant arteries after 48 h exposure to GTN) and aortic ALDH activity. PMID: 21506955
  22. Overexpressed ALDH2 gene may moderate 4-hydroxynonenal-induced neuronal death by regulating caspase-3 protein and reactive oxygen species level in cultured hippocampal neurons. PMID: 20361289
  23. the effects of Mg(2+) on the ALDH2-NADH binding characteristics and enzyme catalysis PMID: 21276780
  24. Data suggest that ALDH2 inhibition may be associated with LV reduced contractility, and mitochondrial impairment aggravated by ALDH2 inhibition might reflect an underlying mechanism which causes cardiac dysfunction in diabetic rats. PMID: 20957334
  25. Ethanol postconditioning upregulated mitochondrial ALDH2 mRNA expression in isolated rat heart. PMID: 21166048
  26. this finding raises the possibility that ALDH2 as the transcriptional regulator might function as a direct sensor of the cellular metabolic state for regulating gene expression at general transcription level. PMID: 21130747
  27. Inhibition of this enzyme is effective in suppressing cocaine-seeking behavior, and may beo of therapeutic value for treating human cocaine addiction and preventing relapse. PMID: 20729865
  28. ALDH2 plays an important role in the bioactivation of glyceryl trinitrate and nitrite in the pulmonary and systemic vascular beds PMID: 20543077
  29. The line difference in AcH aversion is associated more with brain ALDH2 activity than with liver ALDH2 activity. PMID: 14690875
  30. mtALDH overexpression attenuates hyperoxia-induced cell death in lung epithelial cells through reduction of reactive oxygen species, activation of ERK/MAPK, and PI3K-Akt cell survival signaling pathways PMID: 16782756
  31. lipoic acid has a role in the redox regulation of mitochondrial aldehyde dehydrogenase (ALDH-2) activity PMID: 17102135
  32. Increased superoxide production in nitrate tolerance is associated with Nox1 and Aldh2 down-regulation. PMID: 17493633
  33. mitochondrial aldehyde dehydrogenase 2 (ALDH2)was identified as an enzyme whose activation correlates with reduced ischemic heart damage in rodent models PMID: 18787169

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Subcellular Location Mitochondrion matrix
Protein Families Aldehyde dehydrogenase family
Database Links

KEGG: rno:29539

STRING: 10116.ENSRNOP00000001816

UniGene: Rn.101781

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