Rat brain natriuretic peptide,BNP ELISA Kit

Code CSB-E07972r
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name brain natriuretic peptide,BNP
Alternative Names BNP, brain type natriuretic peptide|natriuretic protein
Abbreviation BNP
Uniprot No. P13205
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 125 pg/mL-8000 pg/mL
Sensitivity 31.25 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of rat BNP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:1Average %87
Range %80-92
1:2Average %98
Range %91-105
1:4Average %99
Range %92-110
1:8Average %91
Range %86-98
The recovery of rat BNP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9589-99
EDTA plasma (n=4)9590-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
80002.895 2.898 2.897 2.835
40002.294 2.326 2.310 2.248
20001.327 1.292 1.310 1.248
10000.707 0.689 0.698 0.636
5000.345 0.317 0.331 0.269
2500.196 0.184 0.190 0.128
1250.117 0.114 0.116 0.054
00.064 0.060 0.062  
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
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Target Data

Function Cardiac hormone which may function as a paracrine antifibrotic factor in the heart. Also plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3 (By similarity).
Subcellular Location Secreted
Protein Families Natriuretic peptide family
Tissue Specificity Brain and also in atria, but at much lower levels than ANP.
Database Links

KEGG: rno:25105

STRING: 10116.ENSRNOP00000010778

UniGene: Rn.3835

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