Rat brain natriuretic peptide,BNP ELISA Kit

Code CSB-E07972r
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P13205
Lead Time 3-5 working days
Abbreviation BNP
Target Name brain natriuretic peptide,BNP
Alias BNP, brain type natriuretic peptide|natriuretic protein
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 125 pg/mL-8000 pg/mL
Sensitivity 31.25 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This gene is a member of the natriuretic peptide family and encodes a secreted protein which functions as a cardiac hormone. The protein undergoes two cleavage events, one within the cell and a second after secretion into the blood. The protein s biological actions include natriuresis, diuresis, vasorelaxation, inhibition of renin and aldosterone secretion, and a key role in cardiovascular homeostasis. A high concentration of this protein in the bloodstream is indicative of heart failure. Mutations in this gene have been associated with postmenopausal osteoporosis.
HGNC 7940
RGD 3194
MGI 97368
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat BNP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %87
Range %80-92
1:2Average %98
Range %91-105
1:4Average %99
Range %92-110
1:8Average %91
Range %86-98
Recovery
The recovery of rat BNP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9589-99
EDTA plasma (n=4)9590-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
80002.895 2.898 2.897 2.835
40002.294 2.326 2.310 2.248
20001.327 1.292 1.310 1.248
10000.707 0.689 0.698 0.636
5000.345 0.317 0.331 0.269
2500.196 0.184 0.190 0.128
1250.117 0.114 0.116 0.054
00.064 0.060 0.062  
References
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Function Cardiac hormone which may function as a paracrine antifibrotic factor in the heart. Also plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3 (By similarity).
Subcellular Location Secreted
Protein Families Natriuretic peptide family
Tissue Specificity Brain and also in atria, but at much lower levels than ANP.
Database Links

KEGG: rno:25105

STRING: 10116.ENSRNOP00000010778

UniGene: Rn.3835

Pathway cGMP-PKG signaling pathway

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