Rat calcitonin gene related peptide,CGRP ELISA Kit

Code CSB-E08211r
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
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Product Details

Target Name
calcitonin gene related peptide,CGRP
Alternative Names
Calcb ELISA Kit; Calcitonin gene-related peptide 2 ELISA Kit; Beta-type CGRP ELISA Kit; Calcitonin gene-related peptide II ELISA Kit; CGRP-II ELISA Kit
Abbreviation
CGRP
Uniprot No.
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates
Detection Range
6.25 pg/mL-400 pg/mL
Sensitivity
1.56 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat CGRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 88  
Range % 80-95  
1:2 Average % 94  
Range % 87-101  
1:4 Average % 90  
Range % 86-106  
1:8 Average % 95  
Range % 82-100  
Recovery
The recovery of rat CGRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 89-99  
EDTA plasma (n=4) 94 88-101  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
400 2.742 2.725 2.734 2.562  
200 2.137 2.183 2.160 1.988  
100 1.384 1.481 1.433 1.261  
50 0.863 0.806 0.835 0.663  
25 0.452 0.475 0.464 0.292  
12.5 0.298 0.292 0.295 0.123  
6.25 0.203 0.214 0.209 0.037  
0 0.171 0.173 0.172    
Materials provided
  • A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-Rat CGRP antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
  • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
  • One vial Biotin-labeled CGRP antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
  • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
  • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
  • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
  • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
  • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
  • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
  • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
  • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
  • An instruction manual
Materials not provided
  • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
  • An incubator can provide stable incubation conditions up to 37°C±5°C.
  • Centrifuge
  • Vortex
  • Squirt bottle, manifold dispenser, or automated microplate washer
  • Absorbent paper for blotting the microtiter plate
  • 50-300ul multi-channel micropipette
  • Pipette tips
  • Single-channel micropipette with different ranges
  • 100ml and 500ml graduated cylinders
  • Deionized or distilled water
  • Timer
  • Test tubes for dilution
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Rat CGRP ELISA Kit was designed for the quantitative measurement of Rat CGRP protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 6.25 pg/mL-400 pg/mL and the sensitivity is 1.56 pg/mL.

Citations

Customer Reviews and Q&A

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 Q&A
Q:

Pls inform me the sequence of the standard in this kit

A:
Thanks for your inquiry. This kit is designed against full length protein.Pls refer to https://www.uniprot.org/uniprot/P10093.The standard in it is 293 cell-derived recombinant protein.The sequence of the standard is confidential.Sorry we can not disclose it. Hope you could understand.

Target Background

Function
(From Uniprot)
CGRP induces vasodilation. It dilates a variety of vessels including the coronary, cerebral and systemic vasculature. Its abundance in the CNS also points toward a neurotransmitter or neuromodulator role.
Gene References into Functions
  1. results indicate that calcitonin gene-related peptide-B receptors are present in rat placenta and that receptor levels are higher with gestational age and lower at term labor (Calcb) PMID: 12297551
Subcellular Location
Secreted.
Protein Families
Calcitonin family
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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