Rat diamine oxidase(DAO) ELISA Kit

Code CSB-E12634r
Size 96T,5×96T,10×96T
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
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Product Details

Target Name
D-amino-acid oxidase
Alternative Names
Dao ELISA Kit; Dao1D-amino-acid oxidase ELISA Kit; DAAO ELISA Kit; DAMOX ELISA Kit; DAO ELISA Kit; EC ELISA Kit
Uniprot No.
Rattus norvegicus (Rat)
Sample Types
serum, plasma, cell culture supernates, tissue homogenates
Detection Range
0.78 mIU/mL-50 mIU/mL
0.195 mIU/mL
Assay Time
Sample Volume
Detection Wavelength
450 nm
Research Area
Assay Principle
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of rat DAO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:100Average %85
Range %80-91
1:200Average %93
Range %89-99
1:400Average %87
Range %82-95
1:800Average %101
Range %98-104
The recovery of rat DAO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8581-92
EDTA plasma (n=4)9085-96
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
mIU/ml. OD1OD2AverageCorrected
502.634 2.672 2.653 2.459
252.267 2.298 2.283 2.089
12.51.706 1.765 1.736 1.542
6.251.114 1.156 1.135 0.941
3.120.623 0.672 0.648 0.454
1.560.492 0.480 0.486 0.292
0.780.307 0.322 0.315 0.121
00.193 0.195 0.194  
and FAQs
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days

This Rat DAO ELISA Kit was designed for the quantitative measurement of Rat DAO protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.78 mIU/mL-50 mIU/mL and the sensitivity is 0.195 mIU/mL .

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Target Background

(From Uniprot)
Regulates the level of the neuromodulator D-serine in the brain. Has high activity towards D-DOPA and contributes to dopamine synthesis. Could act as a detoxifying agent which removes D-amino acids accumulated during aging. Acts on a variety of D-amino acids with a preference for those having small hydrophobic side chains followed by those bearing polar, aromatic, and basic groups. Does not act on acidic amino acids.
Gene References into Functions
  1. Plasma DAO activity decreases in response to severe intestinal mucosal injury after 5-FU treatment. PMID: 24005798
  2. Its toxic effect on motor neurons can be mediated by expression in motor neurons and it promotes autophagy. PMID: 24138986
  3. The study showed that the operation of renal ischemia resulted in a 45.49 +/- 8.30% (n = 8) decrease in the DAAO-induced consumption of substrate, indicating a sharp decrease in renal DAAO activity following this acute renal injury. PMID: 21110210
  4. Familial amyotrophic lateral sclerosis is associated with a mutation in D-amino acid oxidase PMID: 20368421
  5. results suggest that an increase in DAO expression in parts of the brain is involved in aberrant D-amino acid metabolism, may be a potential therapeutic target for schizophrenia PMID: 19685198
  6. This result clearly revealed that DAO was indispensable for the process of chiral inversion of d-leucine. PMID: 15026304
  7. These results suggest that the TAA repeats are important for expression of the D-amino-acid oxidase gene. PMID: 18716858

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Subcellular Location
Protein Families
Database Links
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