Name: Recombinant Rat D-amino-acid oxidase (Dao)
Brand: CUSABIO
SKU: CSB-EP006494RA

Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

Dao

Uniprot No.

O35078

Research Area

Metabolism

Alternative Names

Dao; Dao1D-amino-acid oxidase; DAAO; DAMOX; DAO; EC 1.4.3.3

Species

Rattus norvegicus (Rat)

Source

E.coli

Expression Region

1-346aa

Target Protein Sequence

MRVAVIGAGVIGLSTALCIHERYHPAQPLHMKIYADRFTPFTTSDVAAGLWQPYLSDPSNPQEAEWNQQTFDHLQSCLHSPNAEKMGLALISGYNLFRDEVPDPFWKSTVLGFRKLTPSELDMFPDYSYGWFNTSLLLEGKSYLSWLTERLTERGVKFIHRKVASFEEVVRGGVDVIINCTGVWAGALQADASLQPGRGQIIQVEAPWIKHFILTHDPSLGIYNSPYIIPGSKTVTLGGVFQLGNWSELNSVHDHNTIWKSCCQLEPTLKNARIMGELTGFRPVRPQVRLERERLRFGSSSAEVIHNYGHGGYGLTIHWGCAMEAANLFGKILEEKNLSRMPPSHL
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.

Mol. Weight

45.8 kDa

Protein Length

Full Length

Tag Info

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Our Recombinant Rat D-amino-acid oxidase (DAAO) is an essential component in the research arena of Metabolism. The protein, encoded by the 'Dao' gene in Rat species, is integral to the metabolism of D-amino acids, and is known for its catalytic role in oxidizing D-amino acids into their corresponding keto acids. This makes it a valuable tool for understanding metabolic pathways and interactions.

The protein is expressed from E.coli, capturing the full length of the protein from amino acid 1 to 346. In order to increase its versatility in various experimental protocols, the DAAO protein is tagged on the N-terminal with a 10xHis tag and on the C-terminal with a Myc tag, aiding in its purification and detection. Our product boasts a purity greater than 85%, as determined by SDS-PAGE, reinforcing its reliability. You can choose to have the Recombinant Rat DAAO protein in a liquid or lyophilized powder form, allowing for a flexible application in different experimental setups.

Customer Reviews and Q&A

■ Customer Reviews

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■ Q&A

Do you have a rat d-amino acid oxidase protein which has been tested for activity?

Thanks for your inquiry. Regarding this item CSB-YP006494RA, we haven't tested the activity of this protein, so cannot 100% guaranteed. But what we provide is the full length of mature protein, and it is purified under mild condition, it is active in theory.
We can provide this protein derived from four expression systems.
Recombinant Rat D-amino-acid oxidase(Dao)
CSB-YP006494RA >> Yeast
CSB-EP006494RA >> E.coli
CSB-BP006494RA >> Baculovirus
CSB-MP006494RA >> Mammalian cell
Expression region: 1-346aa; Full length
Tag information: EP & BP & MP: N-terminal 10xHis-tagged and C-terminal Myc-tagged；YP: N-terminal 6xHis-tagged
Sequence:

Target Background

Function

Regulates the level of the neuromodulator D-serine in the brain. Has high activity towards D-DOPA and contributes to dopamine synthesis. Could act as a detoxifying agent which removes D-amino acids accumulated during aging. Acts on a variety of D-amino acids with a preference for those having small hydrophobic side chains followed by those bearing polar, aromatic, and basic groups. Does not act on acidic amino acids.

Gene References into Functions

Plasma DAO activity decreases in response to severe intestinal mucosal injury after 5-FU treatment. PMID: 24005798
Its toxic effect on motor neurons can be mediated by expression in motor neurons and it promotes autophagy. PMID: 24138986
The study showed that the operation of renal ischemia resulted in a 45.49 +/- 8.30% (n = 8) decrease in the DAAO-induced consumption of substrate, indicating a sharp decrease in renal DAAO activity following this acute renal injury. PMID: 21110210
Familial amyotrophic lateral sclerosis is associated with a mutation in D-amino acid oxidase PMID: 20368421
results suggest that an increase in DAO expression in parts of the brain is involved in aberrant D-amino acid metabolism, may be a potential therapeutic target for schizophrenia PMID: 19685198
This result clearly revealed that DAO was indispensable for the process of chiral inversion of d-leucine. PMID: 15026304
These results suggest that the TAA repeats are important for expression of the D-amino-acid oxidase gene. PMID: 18716858

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Subcellular Location

Peroxisome.

Protein Families

DAMOX/DASOX family

Database Links

KEGG: rno:114027

STRING: 10116.ENSRNOP00000000881

UniGene: Rn.81058

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